Repetitive transcranial magnetic stimulation ameliorates chronic unpredictable stress?induced depressive?like behaviors in rats model through enhancing hippocampal nuclearfactor?E2?related factor2 antioxidant signaling
10.3760/cma.j.issn.1006-7884.2019.05.007
- VernacularTitle:重复经颅磁刺激通过调节海马的核因子转录因子抗氧化信号通路改善慢性不可预见应激大鼠的抑郁样行为
- Author:
Cuihong ZHOU
1
;
Shanshan XUE
1
;
Jie BAI
1
;
Zhengwu PENG
1
;
Qingrong TAN
1
;
Huaning WANG
1
Author Information
1. 空军军医大学第一附属医院心身科,西安,710032
- Publication Type:Journal Article
- Keywords:
Transcranial Magnetic Stimulation;
Stress;
Nuclearfactor?E2?related factor 2;
Hemeoxygenase?1;
Superoxide Dismutase?1
- From:
Chinese Journal of Psychiatry
2019;52(5):331-338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antidepressive?like effect of repetitive transcranial magnetic stimulation (rTMS) on the rats model exposing chronic unpredictable stress (CUS) and their nuclearfactor?E2?related factor2 (Nrf2) gene expression in hippocampus. Methods (1) SD rats were randomly assigned to the following 3 groups (n=8 for each group):Sham, CUS, CUS+rTMS. The Sham group received only Sham stimulation. The rats in the CUS and CUMS+ rTMS group were subjected to CUS stimulus, followed by 5Hz rTMS or Sham rTMS for 7 days. 24 h after the last stimulation, rats were given a sucrose preference test, an open filed test and a forced swim test. Finally the gene expression of Nrf2, HO?1 and SOD?1 in the hippocampus were determined by real?time PCR and western blot. (2) SD rats were randomly assigned to another 3 groups (n=8 for each group): scramble+CUS, scramble+ CUS+ rTMS and shNrf2+CUS+rTMS. Rats were injected with scramble or shNf2 lentivirus into the dentate gyrus. Two weeks later, all rats were subjected to CUS followed by rTMS or Sham rTMS stimulus for 7 days. Finally rats were given the series behavior tests, and the gene expression of hippocampal Nrf2, HO?1 and SOD?1 were then determined after being sacrificed. Results (1) In comparison with Sham, the rat in CUS group showed significant lower sucrose preference ((77.31 ± 4.69)％vs. (39.33 ± 11.85)％;F2, 21=8.32, P<0.01) and shorter centre time ((52.49±1.07) s vs. (14.70±4.27) s;F2, 21=3.84, P<0.01)) in the open filed test, but longer freezing time in the forced swim test ((24.19 ± 1.07 s vs. (38.70 ± 4.27);F2, 21=10.31, P<0.05);the expression of Nrf2, HO?1 and SOD?1 in the hippocampus was inhibited in the CUS rats. (2) Compared with CUS group, the rat in CUS+rTMS group showed significant better sucrose preference (P<0.05), longer centre time (P<0.05 in open filed test), while shorter freezing time in the forced swim test (P<0.05); the expression of Nrf2, HO?1 and SOD?1 in the hippocampus in CUS+rTMS rats was up?regulated (P<0.05);(3) Rats in shNrf2+CUS+rTMS group showed lower sucrose preference (P<0.05), shorter centre time (P<0.05) in the open filed test and longer freezing time in the forced swim test (P<0.05) and their expression of Nrf2, HO?1 and SOD?1 in the hippocampus (P<0.05) was down?regulated than rats in scramble+CUS+rTMS group. Conclusion rTMS administration may reverse depressive?like behaviors in rats under CUS paradigm and may restore the gene expression of Nrf2, HO?1 and SOD?1 in the hippocampus.
