Effects of edaravone on the expression of AQP4 in rats with open craniocerebral injury coupled with seawater immersion
10.3760/cma.j.issn.1009-6906.2019.05.002
- VernacularTitle:依达拉奉对大鼠海水浸泡开放性颅脑外伤水通道蛋白4表达的影响
- Author:
Bing YANG
1
;
Ming LI
1
;
Xinlong LIU
1
;
Fengfeng XU
1
;
Zhengping XU
1
Author Information
1. 海军特色医学中心, 上海,200052
- Publication Type:Journal Article
- Keywords:
Open craniocerebral injury;
Seawater immersion;
aquaporins 4(AQP4);
Edaravone
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(5):391-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of edaravone on the expression of aquaporins 4 (AQP4) in rats with open craniocerebral injury coupled with seawater immersion. Methods The trauma brain injury rat model was developed by controlled cortical impact ( CCI) . Following injury, the regional trauma brain injury tissue was immersed in the artificial seawater or physiological saline. The trauma brain injury + seawater immersion group and the edaravone intervention group were respectively composed of 63 rats, and 21 rats were designated as the control group. The edaravone intervention group received intraperitoneal edaravone injection (10 mg/kg) after immersion. The pathological changes, brain water content, blood brain barrier infiltration and AQP4 expression level were observed at timepoints of 3, 8 and 24 hours after immersion. Results The semi-quantitative AQP4 expression of the 3-hour seawater immersion group was 0. 222 ± 0. 018, that of the edaravone intervention group was 0. 380 ± 0. 012 and that of the control group was 0. 396 ± 0. 011. The AQP4 expression of the 8-hour seawater immersion group was 0. 228 ± 0. 015 and that of the edaravone intervention group was 0. 162 ± 0. 013. The AQP4 expression of the 24-hour seawater immersion group was 0. 462 ± 0. 013, and that of the edaravone intervention group was 0. 076 ± 0. 017. Variance analysis indicated that there were significant differences in the obtained data, when comparisons were made between the groups (P<0. 05). Conclusion Edaravone could reverse the increasing trend of AQP4 in rats with open craniocerebral trauma injury coupled with seawater immersion within 8 to 24 hours. Its mechanism might be related to the scavenging of free radicals and alleviation of the damage of blood-brain barrier. Changes in AQP4 expression level induced by edaravone might be also an important mechanism in the treatment of open craniocerebral injury coupled with seawater immersion.
