A Subgroup Investigation and Genetic Analysis of Blood Donors in Zhongshan,Guangdong Province,and Research on Preservation Related Blood Group Antigen and Antibody
10.3969/j.issn.1671-7414.2025.06.028
- VernacularTitle:广东中山地区献血员A亚型调查和基因分析及相关血型抗原抗体保存研究
- Author:
Qiao LI
1
;
Ainong SUN
;
Huiyan LIN
;
Jianbao HU
;
Xianguo XU
;
Fei PU
;
Yanting LIAO
;
Yonglun WU
Author Information
1. 中山市中心血站检验科,广东 中山 528400
- Publication Type:Journal Article
- Keywords:
subgroup A;
new allele;
anti-A1;
antigen antibody preservation;
preservative
- From:
Journal of Modern Laboratory Medicine
2025;40(6):154-158,180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of A subgroups in the A and AB blood type populations among voluntary blood donors in Zhongshan,study the antigen-antibody characteristics of A subgroups,establish a local A subgroup database,and support the development of precision medicine.Methods ABO subgroup screening was performed using the microplate method.Specimens negative for monoclonal anti-A1 reactivity underwent sequencing of exons 1～7 of the ABO gene to confirm genotypes.Cryopreservation and thawing of glycerolized subgroup red blood cells(RBCs),as well as preservation efficacy of concentrated human-derived antibodies with preservatives,were studied.Results Among 1 212 blood donor specimens,28 subgroup specimens were identified,with a prevalence of 1.54%(15/971)in blood type A and 5.39%(13/241)in blood type AB.Sequencing of 10 specimens revealed 7 ABO genotypes:ABO*A2.01/O.01.02(2 cases),ABO*A1.02/B.01(3 cases),ABO*BA.02/O.01.02,ABO*AW.31.02-05/A2.05,ABO*A2.05/B.01,ABO*A2new/O.01.01,and ABO*A1.02/O.01.01(1 case each).Additionally,one rare allele mutation(c.700C>G)and one novel allele mutation(c.203G>T)(GenBank accession number:PQ152337)were identified.Human anti-A1 antibodies with a titer of 8 were successfully concentrated.Optimal preservation conditions included 0.1%preservative concentration and cryopreserved subgroup RBCs stored at 4℃for 3 days post-thaw.Conclusion The predominant A subgroups in Zhongshan donors are A2 and B(A).A preliminary database for A2 and A2B subgroups is established,along with the discovery of a novel ABO allele mutation.Cryopreservation with glycerol,PEG antibody concentration,and ProClin 300 preservative demonstrate effective applications in preserving ABO blood group antigens and antibodies.
