Value of HGF and TGF-β1 levels in serum and bronchoalveolar lavage fluid in the diagnosis of early stage non-small cell lung cancer
10.3760/cma.j.issn.1009-6906.2019.05.013
- VernacularTitle:支气管肺泡灌洗液及血清中HGF、TGF-β1水平对早期非小细胞肺癌的诊断价值
- Author:
Wei DING
1
;
Jiaru HUANG
1
;
Ming LIU
1
;
Xiaoli ZHOU
1
;
Yunfeng ZHAO
1
Author Information
1. 上海市浦东新区浦南医院呼吸科, 上海,200125
- Publication Type:Journal Article
- Keywords:
Hepatocyte growth factor;
Transforming growth factor-β1;
Early stage non-small cell lung cancer;
Bronchoalveolar lavage fluid
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2019;26(5):435-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the value of hepatocyte growth factor ( HGF ) and transforming growth factor-β1 (TGF-β1) in the diagnosis of early stage non-small cell lung cancer (ES-NSCLC) through detection of their levels in serum and bronchoalveolar lavage fluid ( BALF ) . Methods Serum and BALF samples were collected from 48 cases of ES-NSCLC ( at stage 1 and 2 ) , 45 cases of medium and advanced NSCLC (at stage 3 and 4), 42 cases of benign pulmonary nodule and 30 controls for the study. The levels of HGF and TGF-β1 were respectively detected by ELISA, and their differences were analyzed between the 4 groups. The relationship between HGF and TGF-β1 levels and tumor size, differentiation and pathological type was further analyzed, and sensitivity and specificity in the diagnosis of ES-NSCLC was evaluated in the study. Results The levels of HGF and TGF-β1 in BALF of the ES-NSCLC group were significantly higher than those in the benign pulmonary nodule group and the normal control group (P <0. 05). HGF and TGF-β1 serum levels in the ES-NSCLC group were higher than those in the benign pulmonary nodule group and the control group, however, no statistical significance could be seen when comparisons were made between them ( P >0. 05). Serum HGF and TGF-β1 levels in the BALF of the medium and advanced NSCLC group were significantly higher than those in the ES-NSCLC group (P<0. 05). The areas under the curve (AUC) in the BALF of HGF and TGF-1 were respectively 0. 754 and 0. 839. When the levels of 59. 32pg/ml and 98. 52pg/ml were selected as the optimal cutoff values according to the Jordan index maximum method, the sensitivity of HGF and TGF-β1 in the diagnosis of ES-NSCLC were respectively 41. 7％ and 81. 3％, and specificity were respectively 96. 3％ and 77. 8％. Furthermore, in the patients with adenocarcinoma or tumor size larger than 3cm in diameter, higher expression levels could be detected. Conclusion High expression levels of HGF and TGF-β1 could be detected in the BALF of the patients with ES-NSCLC with high specificity. For this reason, it has certain clinical value in the accessory diagnosis of ES-NSCLC.
