Relationship between SIRT1 and tau protein during neuroinflammation induced by cardiopulmonary bypass in rats
10.3760/cma.j.cn131073-20240807-00809
- VernacularTitle:体外循环致大鼠神经炎症时SIRT1与tau蛋白的关系
- Author:
Yunfu CHEN
1
;
Yi SHAO
1
;
Ke JIANG
1
;
Jie LYU
1
;
Jing SHI
1
Author Information
1. 贵州医科大学附属医院麻醉科，贵州 550004
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary bypass;
Inflammation;
Sirtuin 1;
tau Proteins
- From:
Chinese Journal of Anesthesiology
2025;45(8):966-971
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the relationship between NAD + -dependent protein deacylase sirtuin-1 (SIRT1) and tau protein during cardiopulmonary bypass (CPB)-induced neuroinflammation in rats. Method:Thirty-six healthy adult male Sprague-Dawley rats, weighing 250-300 g, were used in this study. Eighteen rats were divided into 3 groups ( n=6 each) using a random number table method: sham operation group (C1 group), CPB1 group and tau protein inhibitor methionine+ CPB group (methionine+ CPB group). Another 18 rats were divided into 3 groups ( n=6 each) using a random number table method: sham operation group (C2 group), CPB2 group and SIRT1 agonist SRT1720+ CPB group (SRT+ CPB group). Methionine+ CPB group received oral methionine 10.5 mg/kg once a day for 7 consecutive days. SRT1720 10 mg/kg was intraperitoneally injected once a day for 5 consecutive days in SRT+ CPB group. The model of CPB was developed after the end of drug administration. Rats were weaned off the bypass system after 1 h of circulatory support and sacrificed, and brain tissues were harvested for determination of the contents of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and IL-4 (using enzyme-linked immunosorbent assay), expression of CD32, ARG1, SIRT1, tau protein and acetylated tau protein (ac-tau) (by Western blot), and expression of CD32, ARG1, SIRT1 and tau mRNA (by quantitative real-time polymerase chain reaction). Results:Compared with C1 group, the contents of TNF-α and IL-6 were significantly increased, the content of IL-4 was decreased, the expression of tau and CD32 protein and mRNA was up-regulated, and the expression of ARG1 protein and mRNA was down-regulated in CPB1 group ( P<0.05). Compared with CPB1 group, the contents of TNF-α and IL-6 were significantly decreased, the content of IL-4 was increased, the expression of tau and CD32 protein and mRNA was down-regulated, and the expression of ARG1 protein and mRNA was up-regulated in M+ CPB group ( P<0.05). Compared with C2 group, the contents of TNF-α and IL-6 were significantly increased, the content of IL-4 was decreased, the expression of ac-tau and tau and CD32 protein and mRNA was up-regulated, and the expression of SITR1 and ARG1 protein and mRNA was down-regulated in CPB2 group ( P<0.05). Compared with CPB2 group, the contents of TNF-α and IL-6 were significantly decreased, the content of IL-4 was increased, the expression of ac-tau and tau and CD32 protein and mRNA was down-regulated, and the expression of SITR1 and ARG1 protein and mRNA was up-regulated in SRT+ CPB group ( P<0.05). Conclusions:The down-regulated SIRT1 expression in brain tissues after cardiopulmonary bypass can inhibit deacetylation of tau protein, promote M1 polarization of microglia, inhibit M2 polarization, and ultimately induce neuroinflammation in rats.
