Progress in establishment and application of the reverse genetics system for Orthobunyaviruses
10.3760/cma.j.cn112866-20250627-00133
- VernacularTitle:正布尼亚病毒反向遗传学系统的建立及其应用进展
- Author:
Wei CHEN
1
;
Nanjie REN
;
Yashuang GUO
;
Fei WANG
;
Zhiming YUAN
;
Han XIA
Author Information
1. 中国科学院武汉病毒研究所，武汉 430071
- Publication Type:Journal Article
- Keywords:
Orthobunyavirus;
Reverse genetics system;
Infectious clone;
Virus rescue
- From:
Chinese Journal of Experimental and Clinical Virology
2025;39(5):660-670
- CountryChina
- Language:Chinese
-
Abstract:
The orthobunyavirus is a major group of mosquito-borne viruses，mainly prevalent in the Americas，Europe，and Africa. It can cause severe human diseases（e.g.，encephalitis，hemorrhagic fever）and livestock abortions. Reverse genetics，as a powerful tool for viral genome research，has been widely applied to study viral gene functions，develop vaccines，and screen antiviral drugs. This review systematically presents the evolutionary history and applications of the reverse genetics system for orthobunyaviruses，providing critical insights into studies on the infection and transmission mechanisms of these viruses，as well as the development of novel vaccines and therapeutic agents.
