Value of Detection of Serum Exosome hsa_circ_0084443 in Diabetic Foot Ulcer Patients as Early Clinical Diagnosis
10.3969/j.issn.1671-7414.2025.06.016
- VernacularTitle:糖尿病足溃疡患者血清外泌体hsa_circ_0084443水平检测对早期临床诊断的价值研究
- Author:
Haozhe FU
1
;
Jichang WANG
;
Yuanhang BI
Author Information
1. 宝鸡市中心医院血管外科,陕西 宝鸡 721000
- Publication Type:Journal Article
- Keywords:
hsa_circ_0084443;
exosome;
diabetic foot ulcer;
early diagnosis;
microarray chip
- From:
Journal of Modern Laboratory Medicine
2025;40(6):86-89,115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the feasibility of serum exosome hsa_circ_0084443 as a biomarker for early diagnosis of diabetic foot ulcer.Methods GSE114236 chip data from the Gene Expression Omnibus(GEO)database was downloaded to screen the differentially expressed circular DNA(circRNA).From December 2019 to August 2021,44 T2DM patients with diabetic foot ulcer(DFU)who received treatment at Baoji Central Hospital were included as the study subjects,all patients had Wagner grades 1～2.In addition,185 newly diagnosed T2DM patients without comorbidities(DM group)were included as controls during the same period.Serum samples from all participants were collected for extracting purified serum exosomes.The expression of serum exosomal hsa_circ_0084443 was detected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR),and the differences among different groups was compared.Spearman rank correlation analysis was used to investigate the relationship between serum exosomal hsa_circ_0084443 and Ankle-Brachial Index(ABI).Receiver operating characteristic(ROC)curves was used to analyze the efficacy of serum exosomal hsa_circ_0084443 in diagnosing early DFU.Results By analyzing the GSE114236 dataset,it was found that hsa_circ_0084443 was the circRNA with the highest differential expression.In the whole cohort analysis,the expression of serum exosome hsa_circ_0084443 in DFU group was significantly higher than that in DM group[1.18(0.84,2.06)vs 0.73(0.51,1.16)],and the differences was statistically significant(Z=-4.219,P<0.001).The expression level of serum exosome hsa_circ_0084443 in DFU group was negatively correlated with ABI(r=-0.420,P=0.005).After adjusting for age,smoking history and creatinine,the increased expression level of serum exosome hsa_circ_0084443 was still an independent risk factor for DFU(P＜0.001).Finally,the area under ROC curve(AUC)of serum exosome hsa_circ_0084443 for the diagnosis of early DFU was 0.705.Conclusion Serum exosome hsa_circ_0084443 is highly expressed in DFU patients and can be used as an important marker for early diagnosis of DFU.
