Assessment of the Feasibility of Bloodstream Infection Diagnosis with A 4-Day Incubation Time Setting in the BacTALERT VIRTUO Blood Culture System
10.3969/j.issn.1671-7414.2025.06.003
- VernacularTitle:BacTALERT VIRTUO血培养系统设定4天孵育时间对于血流感染诊断的可行性评估
- Author:
Yan MA
1
;
Chunmei ZHOU
;
Shenglei HUANG
;
Wei GUO
;
Beili WANG
;
Boshen PAN
Author Information
1. 复旦大学附属中山医院检验科,上海 200032
- Publication Type:Journal Article
- Keywords:
blood culture;
pathogens;
time to detection;
bloodstream infection
- From:
Journal of Modern Laboratory Medicine
2025;40(6):12-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the impact of setting a 4-day incubation time on the detection performance of bloodstream infection(BSI)pathogens by the BacTALERT VIRTUO blood culture system(VIRTUO).Methods Retrospectively collecting blood culture(BC)results from Zhongshan Hospital,Fudan University,from June 2021 to December 2023,and comparing the time-to-detection for clinically significant microorganisms.Results A total of 87 052 BC bottles were collected,of which 7 167(8.23%)were positive.95%of aerobic and anaerobic bottles were reported positive at 65.67 h and 91.2 h respectively.The average(median)time-to-detection for Staphylococcus aureus(S.aureus)was 21.76(16.08)h,Klebsiella pneumoniae(KP)was 15.32(11.65)h,Escherichia coli(E.coli)was 16.14(10.87)h,and Candida species was 35.2(28.5)h.Only 213 bottles(0.24%)were reported positive after 4 days of incubation;among them,88 bottles(41.31%)cultured Propionibacterium acnes(P.acnse).Medical chart reviews for BC reported positive after 96 h(4 days)showed no clinical significance.Simulated growth experiments were conducted on 11 fastidious bacteria,and the median detection time was only 10.8 h,indicating that an incubation time of 4 days is sufficient for fastidious bacteria.Conclusion A 4-day incubation is adequate for VIRTUO system.Reporting 1 day earlier to the doctors can reduce the positive rate of contaminants and the use of antimicrobial agents.
