Effects of hyperbaric oxygen combined with Adriamycin on HepG2 cell apoptosis and immunomodulatory factors in transplanted hepatocellular carcinoma of nude mice
10.3760/cma.j.cn311847-20210516-00155
- VernacularTitle:高压氧联合阿霉素处理对裸鼠肝癌移植瘤HepG2细胞凋亡及免疫调节因子的影响
- Author:
Peng YAO
1
;
Xiuli WANG
;
Xiaozheng LI
;
Haimin LI
;
Jiayong NING
;
Jiasui CHAI
;
Jiejie DONG
Author Information
1. 运城市中心医院肝胆外科，运城 044000
- Publication Type:Journal Article
- Keywords:
Hyperbaric oxygen;
Liver cancer;
Apoptosis;
Immunomodulatory factor;
Adriamycin
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2022;29(3):304-307,316
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of adriamycin（Adc）combined with hyperbaric oxygen（HBO）on the apoptosis of HepG2 cells and immunomodulatory factors in transplanted hepatocellular carcinoma（HCC）tissues in nude mice.Methods:The model of transplanted HCC was established in 20 nude mice，then the mice were divided into four groups：control group，HBO group，Adc group，and HBO + Adc group，with five mice in each group. The control group was reared normally. The HBO group was treated with HBO at 0.20 MPa for 1 h；the Adc group was treated with 10 mg/kg of Adc by gavage；the HBO + Adc group was treated with HBO at 0.20 MPa for 1 h and then given 10 mg/kg of Adc by gavage. All groups were treated once every other day for 21 days. After drug withdrawal，the tumors were dissected from the sacrificed mice，of which the tumor volume and weight were measured. The apoptosis of HepG2 cells was detected by TUNEL. The changes of apoptosis-related proteins in HepG2 cells were detected by western blotting. The changes of the contents of immunomodulatory factors in transplanted tumor tissues of nude mice were detected by ELISA.Results:After 21 days，the tumor volume and weight in the HBO + Adc group were lower than those in the other three groups（ P<0.05）. The proportion of apoptotic cells in the transplanted tumor tissues of nude mice of the HBO + Adc group was the highest. In the HBO + Adc group，the protein expression levels of Bax，caspase-3，and Cytochrome C were significantly increased；the protein expression of Bcl-2 was significantly reduced（ P<0.01）；and the IL-6 was significantly reduced；while the IL-18 and IFN-γ were significantly increased（ P<0.05）. Conclusion:The Adc combined with HBO can promote apoptosis of HepG2 cells in transplanted HCC tissues in nude mice，and effectively regulate the immunomodulatory factors to promote the recovery of the body’s immune function，suggesting obvious sensitizing and detoxifying effects.
