Influence of high +Gz environment on receptor activator of nuclear factor κB ligand and osteoprotegerin mRNA expression in the peri-implant bone tissue
10.3760/cma.j.issn.1007-6239.2018.03-04.013
- VernacularTitle:高+Gz暴露对种植体周围骨组织核因子κB受体活化因子配体及骨保护素mRNA表达的影响
- Author:
Xiaoru ZHU
1
;
Tianzheng DENG
1
;
Jianliang PANG
1
;
Bing LIU
1
;
Jie KE
1
Author Information
1. 空军特色医学中心口腔科, 北京,100142
- Publication Type:Journal Article
- Keywords:
Acceleration;
Rabbits;
Dental implant;
Receptor activator of nuclear factor-kappa B ligand;
Osteoprotegerin
- From:
Chinese Journal of Aerospace Medicine
2018;29(3):231-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of high + Gzenvironment on receptor activator of nuclear factor κB ligand (RANKL) and osteoprotegerin (OPG) messenger RNA (mRNA ) expression in the peri-implant bone tissue by an animal model. Methods Thirty New Zealand white rabbits were randomly and averagely divided into 3 control groups and 3 experimental groups corresponding to the investigation for 3 weeks ,5 weeks and 12 weeks respectively.Rabbits′ mandible incisors of both sides were extracted and im planted with 1 implant in each socket immediately.After 1 week of rest ,the experimental rabbits were exposed to high + Gzenvironment 3 times a week (Monday ,Wednesday ,Friday) ,The exposure scheme choose the + Gzprofile with 4～9 G for 10～45 s with 1 G/s onset rate and with the intervals of 1 min.The rabbits of control groups were not exposed to + Gzenvironment and normally fed.One control group and one experimental group rabbits were respectively sacrificed at the 3rd week (+Gzexposure for 2 weeks) , 5th week (+Gzexposure for 4 weeks) and 12th week (+Gzexposure for 4 weeks and normal feeding for 7 weeks) after the implanting surgery.The specimens were obtained to find the mRNA expression of RANKL and OPG by real-time polymerase chain reaction (RT-PCR) examination. Results The mRNA expression of RA NKL and OPG were the highest 3 weeks after the surgery in both groups , and then decreased gradually.The RANKL mRNA expression and the RANKL/OPG ratio of the experimental group were significantly higher than those of the control group 3 weeks after the surgery (P＜0.05).Five weeks after the surgery ,the OPG mRNA expression of the experimental group was significantlylowerthanthatofthecontrolgroup(P＜0.05),buttheRANKLmRNAexpressionand RANKL/OPG ratio were still higher than that of control group (P＜0.05).Twelve weeks after the surgery ,the differences on RANKL and OPG mRNA expressions was not obvious between two groups. Conclusions High +Gzexposure could increase the expression of RA N K L ,raise RA N K L/OPG ratio and promote bone resorption ,but may be not conducive to implant bone bonding.
