Continuous observation on the Light criteria of pleural effusion in hypothermic rats induced by seawater immersion
10.3760/cma.j.issn.1009-6906.2018.03.001
- VernacularTitle:Light标准对海水浸泡体温过低症模型大鼠胸水性质的连续观察
- Author:
Jianbo HUAN
1
;
Lina CHEN
;
Zhihai HAN
;
Licheng SONG
;
Hao CHENG
;
Yuanyuan QIAO
;
Dajin ZHANG
;
Chenghe SHI
Author Information
1. 海军总医院呼吸与危重症医学科,北京,100048
- Publication Type:Journal Article
- Keywords:
Seawater immersion;
Hypothermia;
Rats;
Rewarming;
Pleural effusion;
Light criteria
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2018;25(3):129-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dynamic changes in the nature of the pleural effusion via Light criteria in hypothermic rats induced by seawater immersion and analyze possible mechanism involved.Methods One hundred male Sprague-Dawley rats were randomly divided into the normal control group (without any treatment) and hypothermia group exposed to 20 ℃ seawater for 24 hours.Then,the hypothermia group was sub-divided into the passive rewarming groups 1,2,3 and 4 and warm water bath active rewarming groups 1,2,3 and 4,each consisting of 10 animals.The passive rewarming groups had passive rewarming after exposure to 20 ℃ seawater for 24 hours,while the active rewarming groups had warm water bath rewarming following exposure to 20 ℃ seawater for 24 hours.Then,all the animals in the sub-groups were executed 0,3,6 and 12 hours after rewarming.Serum,pleural effusion,total protein (TP) in pleural effusion,concentrations of lung homogenate lactate dehydrogenase (LDH) were measured,and Light criteria were calculated.Results There was no significant pleural effusion in the normal rats.LDH level in hypothermia-induced pleural effusion was higher than that in normal serum LDH,pleural effusion/total serum protein ratio (TPR) was lower than 0.5,and lactate dehydrogenase ratio (LDHR) was lower than 0.6.After rewarming,the amount of hypothermia-induced pleural effusion decreased gradually,while the levels of TPR and LDHR increased gradually.However,changes of LDH in pleural effusion were different with those in serum and lung homogenate.The warm water bath rewarming in the absorption of hypothermic pleural effusion was faster than passive rewarming,Warm water bath rewarming seemed to promote absorption of hypothermia-induced pleural effusion,but without statistical significance.Conclusions The 3 values of the pleural effusion criteria in hypothermic rats continuously increased following rewarming and reached the standards of effusion fluid,which did not necessarily reflect the seriousness of inflammatory pleural damage.The possible mechanism involved might be associated with the decrease of pleural effusion after rewarming,and water absorption by the body might be greater than protein absorption.
