The Mechanobiological Response of Immature Dendritic Cells to Nanomorphologies with Different Crystalline Phases on Titanium Surfaces
10.16156/j.1004-7220.2025.05.025
- VernacularTitle:未成熟树突状细胞对钛表面不同晶相纳米形貌的力学生物学响应
- Author:
Zhaoyajing LUO
1
;
Yi WU
;
Hong CHEN
;
Jin CHEN
;
Zuquan HU
;
Zhu ZENG
;
Yun WANG
Author Information
1. 贵州医科大学生物与工程学院(健康医药现代产业学院),贵州省普通高等学校免疫细胞与抗体工程研究中心,贵阳 550000
- Publication Type:Journal Article
- Keywords:
titanium;
crystalline phase structure;
dendritic cells;
mechanobiological response
- From:
Journal of Medical Biomechanics
2025;40(5):1272-1280
- CountryChina
- Language:Chinese
-
Abstract:
Objective To elucidate the regulatory effects of titanium surface modification on the immune function of immature dendritic cells(imDCs),different crystalline nanomorphologies were constructed on titanium surface to investigate the mechanobiological response of imDCs to nanomorphologies with different crystalline phases.Methods Nanomorphologies with different crystalline phases were constructed on the titanium surface by anodic oxidation and calcination.The changes of the cytoskeleton F-actin,cell adhesion and morphology of imDCs cultured on nanomorphologies with different crystalline phases were observed by fluorescence staining.The relative gene expression of adhesion molecules was detected by quantitative real-time PCR.The migration behaviors of imDCs were observed using real-time live-cell imaging,and the membrane fluidity was detected by fluorescence polarization.Results Nanomorphologies with different crystalline phases,namely amorphous phase,anatase and rutile,were obtained on the titanium surface by anodic oxidation and calcination.The cytoskeleton of imDCs on nanomorphologies with different crystalline phases was remodeled.The spreading area of cells on anatase crystalline phase was relatively small,which was(353.3±148.5)μm2.The number of adherent cells was the largest,which was 587±132.The expression of adhesion molecules such as CD11a,integrin β2,ICAM1,and VCAM1 were also increased in cells which cultured on anatase crystalline phase.The imDCs cultured on anatase crystalline phase were equipped with strong migration ability.The accumulative migration distance was(383.6±177.7)μm,and the Euclidean migration distance was(51.82±50.13)μm.The membrane fluidity was relatively weak,and the fluorescence polarization was 0.348 5±0.041 8.Conclusions imDCs can respond to nanomorphologies with different crystalline phases on the titanium surface and exhibit different biomechanical behaviors.The results might provide a theoretical basis for the design of titanium biomaterials with immunomodulatory functions.
