Optimization of the liquid fermentation medium of the marine petroleum-degrading bacteria Acinetobacter sp.Y9
10.3760/cma.j.issn.1009-6906.2018.01.008
- VernacularTitle:海洋石油降解菌Acinetobacter sp.Y9发酵培养基的优化
- Author:
Xue TANG
1
;
Dengyong HOU
;
Xianrong SHEN
;
Ying HE
;
Qun LUO
;
Qingrong WANG
Author Information
1. 上海海洋大学食品学院,上海,201306
- Publication Type:Journal Article
- Keywords:
Acinetobacter sp.Y9;
Medium;
Fermentation;
Screen;
Optimization
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2018;25(1):27-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of the types and concentrations of major medium components on the growth of the marine oil-degrading bacterial strain Acinetobacter sp.Y9.Methods Carbon,nitrogen and phosphorus sources in the medium of Y9 fermentation were screened and optimized by using single factor and orthogonal experiments,and the optimized medium was applied for initial fermentation of Acinetobacter sp.Y9 in the 10 L fermentor.Results After optimization,the optimal fermentation medium of Y9 included 1％ glucose,2％ peptone,0.1 mol/L phosphate buffer solution(Na2HPO4 · 12H2O + KH2PO4),0.05％ NaCl2,0.001％ CaCl2 and 0.019 ％ MgCl2.The value of OD~ of Y9 reached 8.24 after cultivation in the optimized medium in the 10 L fermentor for 12 hours.Conclusions The optimized medium could be used in the pilot fermentation production of Y9 strain,which might lay a solid foundation for large scale fermentation production of Y9.
