Entrainment of different light regimens on the circadian rhythm of peripheral blood immune cells in mice
10.3760/cma.j.issn.1009-6906.2017.06.006
- VernacularTitle:不同光制对小鼠外周血免疫细胞近日节律的导引研究
- Author:
Qingmin WANG
1
;
Qiuhong LIU
1
;
Fenzhou SHI
1
;
Kehua LI
1
;
Shenglong DAI
1
;
Yongjie YAO
1
;
Ying TANG
1
Author Information
1. 海军军医大学海军医学研究所航空医学研究室, 上海,200433
- Publication Type:Journal Article
- Keywords:
Circadian rhythm;
Entrainment;
Mouse;
Peripheral blood;
Immune cell
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2017;24(6):440-444
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the entrainment effect of different light regimens on the circadian rhythms of peripheral blood immune cells in mice .Methods One hundred and forty-four male C57BL/6 mice were kept under the 12 h-light and 12 h-dark cycle for 4 weeks.Then, the animals were randomly divided into 3 groups.The 24 animals in group 1 were treated with the former light regimen , the 60 animals in group 2 were kept in the light regimen of phase advance 12 hours, simulating eastward flight, and another 60 animals in group 3 were kept in the light regimen of phase delay 12 hours, simulating westward flight.Peripheral blood samples of the animals in group 1 were collected respectively one day before and 21 days after changes of light regimens, and blood samples of the animals in group 2 and 3 were collected on different days (i.e.day 2, 4, 7, 14 and 21) after changes of light regimens .Peripheral blood samples were taken at 4 different timepoints in 24 hours ( i.e.9 :00, 15:00, 21 :00 and 3 :00).Detections were made in immune cells , including the numbers of leukocytes, the percentage of lymphocytes , the percentage of CD4 +lymphocytes, the percentage of CD8 +lymphocytes, as well as monocyte and granulocyte counts .Finally, the circadian rhythms of differentimmune cells were evaluated in the animals of different groups .Results The immune cells in the C57BL/6 mice displayed different circadian rhythms under the 12 h-light and 12 h-dark cycle for 4 weeks.Under the new light regimens, the circadian rhythms of immune cells were entrained .It took 2 weeks for the animals of the simulated westward flight phase delay group to entrain the circadian rhythm , while the rate of entrainment for the animals of the simulated eastward flight phase delay group was 3 weeks.Conclusions Under the 12 h-light and 12 h-dark cycle, immune cells in the peripheral blood displayed a rise and fall circadian rhythm , which might facilitate the balance of the immune system .However, under the new light regimens, the circadian rhythms of different immune cells in the peripheral blood were entrained , with the entrainment rate of immune cells in the phase delay group being faster than that of the phase advance group .
