Effects of salinity and lipopolysaccharide on the mode mice of acute respiratory distress syndromes induced by seawater drowning
10.3760/cma.j.issn.1009-6906.2017.06.008
- VernacularTitle:盐度及脂多糖对海水淹溺型急性呼吸窘迫综合征模型小鼠的影响
- Author:
Xuefeng LIU
1
;
Zhaofen LIN
1
Author Information
1. 海军军医大学附属长征医院, 上海,200003
- Publication Type:Journal Article
- Keywords:
Salinity;
Lipopolysaecharide;
Acute respiratory distress syndromes;
Seawater drowning
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2017;24(6):448-451
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of natural seawater and lipopolysaccharide (LPS) on acute respiratory distress syndromes (ARDS) induced by seawater drowning .Methods Fifty male Kunming mice were randomly divided into 5 groups, each consisting of 10 animals.They were: the blank control group (group A); the normal saline (NS) group (group B), which was injected 4ml/kg NS into the airway passage;the low salinity artificial seawater group (group C), which was injected 4ml/kg artificial seawater at a salinity of 1.67％ into the airway passage; the LPS antagonism group (group D), which was first given LPS injection (2 mg/kg) in the abdominal cavity and then 4 ml/kg natural seawater in the airway passage at day 8; the seawater group (group E), which was injected 4 ml/kg of natural seawater in the airway passage .Four hours after injection of seawater, the animals were sacrificed, LPI and wet /dry ratio (W/D) were detected, bronchis-alveolar lavage fluid (BALF) was collected, and levels of neutrophi , TNF-α, IL-1βwere detected.Results As compared with those of the blank control group , the levels of LPI, W/D, neutrophil[group D:(1.69 ± 0.09) ×106 /L,group E:(2.11 ±0.12) ×106 /L], TNF-α[group D:(202.31 ±28.76) ng/L, group E:(233.72 ±26.41) ng/L] and IL-1β[group D:(178.16 ±24.85) ng/L,group E:(206.38 ±20.56) ng/L] in the groups B,C D and E were all significantly elevated 4 hours after injection of seawater .The levels of group E were >those of group C >those of group B >those of group A, and statistical significance could be seen when comparisons were made between the groups (P <0.05).All the values in group E were higher than those in group D, and there was statistical significance in the levels of neutrophi , TNF-αand IL-1β, when comparisons were made between them.Conclusions Both the salinity and lipopolysaccharide content of seawater were the main causative agents of acute respiratory distress syndromes after seawater drowning .
