Analgesic effect of methane rich saline in monoarthritis model rat

VernacularTitle:甲烷饱和盐水对单关节炎大鼠镇痛作用的研究
Author: Shuzhuan ZHOU ¹ ; Yalan ZHOU ¹ ; Haoling LI ¹ ; Hua XU ¹
Author Information

1. 海军军医大学附属长海医院麻醉科, 上海,200433
Publication Type:Journal Article
Keywords: Monoarthritis; Methane; Methane rich saline; Neuro-inflammation; Oxidative Stress
From: Chinese journal of nautical medicine and hyperbaric medicine 2017;24(5):385-389
CountryChina
Language:Chinese
Abstract: Objective To investigate the analgesic effect of methane rich saline (MS) on monoarthritis (MA) rat and its possible mechanism.Methods We utilized Complete Freund's Adjuvant (CFA) to induce MApain model,and observed the effect of MS on MA rats by behavior assess,western blot,enzyme linked immunosorbent assay(ELISA),and oxidative stress assay.Results Intraperitoneal injections of MS attenuated MA-induced mechanical allodynia.MS treatments inhibited the activation of microglia and astrocytes,decreased IL-1β and TNF-α production in the spinal cord on day 10 post-operation.Additionally,MS also suppressed MA-induced oxidative stress (MDA),and protected the antioxidant capacity (SOD and CAT) in spinal cord dorsal horn.Conclusions Our findssuggested the analgesic effect of MS on monoarthritis pain,and imply antineuroinflammation and anti-oxidative stress as the associated mechanisms.