lncRNA FGD5-AS1 regulates gastric cancer cell migration and invasion via the miR-133a-3p/SPAG5 axis
10.12007/j.issn.0258-4646.2025.05.004
- VernacularTitle:lncRNA FGD5-AS1调节miR-133a-3p/SPAG5轴对胃癌细胞迁移和侵袭的影响
- Author:
Yuanhang CHEN
1
;
Lang HE
;
Mao TAN
;
Yi XU
;
Xia LI
Author Information
1. 成都市第五人民医院 肿瘤科,成都 611130
- Publication Type:Journal Article
- Keywords:
long noncoding RNA FGD5-AS1;
miR-133a-3p/SPAG5 axis;
gastric cancer;
migration;
invasion
- From:
Journal of China Medical University
2025;54(5):401-406
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the long noncoding RNA(lncRNA)FGD5-AS1 on the migration and invasion of gas-tric cancer cells by regulating the miR-133a-3p/SPAG5 axis.Methods Quantitative real-time PCR was used to detect FGD5-AS1,miR-133a-3p,and SPAG5mRNA expression in gastric cancer tissues and adjacent non-tumor tissues,the normal human gastric epithelial cell line GES-1,and gastric cancer cell lines(MKN-28 and NCI-N87,HGC-27,and AGS).Cell counting kit-8 method and 5-ethynyl-2-de-oxyuridine(EdU)staining were performed to detect cell proliferation.Transwell assays were performed to assess cell invasion.A scratch assay was performed to detect the migration ability.Western blotting was performed to detect the expression of the proliferative proteins Ki-67,SPAG5,migration,invasion enhancer factor 1(MIEN1),and matrix proteinase-9(MMP-9).A double-luciferase assay was used to confirm the relationship between miR-133a-3p,FGD5-AS1,and SPAG5expression.Results FGD5-AS1 and SPAG5mRNA levels were significantly elevated in gastric cancer tissues and cell lines,whereas miR-133a-3p was significantly reduced(P<0.05),with the most significant gene changes observed in MKN-28 cells(P<0.05).Compared with the blank and siNC groups,FGD5-AS1,SPAG5mRNA,EdU-positive rate,proliferative,invasive,and migratory abilities,Ki-67,MIEN1,SPAG5,and MMP-9 expression in the siFGD5-AS1 group decreased,and the expression of miR-133a-3p increased(P<0.05).Compared to the siFGD5-AS1+inhibitor NC group,the expres-sion of SPAG5mRNA;proliferative,invasive,and migratory abilities;and Ki-67,MIEN1,SPAG5,and MMP-9 expression in the siFGD5-AS1+miR-133a-3p inhibitor group increased,and the expression of miR-133a-3p decreased(P<0.05).Conclusion Interference with lncRNA RNA FGD5-AS1 can inhibit the migration and invasion of gastric cancer cells by upregulating the miR-133a-3p/SPAG5 axis.
