Therapeutic effect of sulforaphane on neuronal mitochondrial damage of brain tissue in rats with acute carbon monoxide poisoning
10.3760/cma.j.issn.1009-6906.2018.04.002
- VernacularTitle:莱菔硫烷对急性一氧化碳中毒大鼠脑组织神经细胞线粒体损伤的治疗作用
- Author:
Xiaoyu DING
1
;
Li WANG
;
Weikang BI
;
Hai KANG
;
Mingjun BI
;
Yong ZOU
;
Qin LI
Author Information
1. 266021 山东省青岛,青岛大学医学部中西医结合中心;青岛大学附属烟台毓璜顶医院中西医结合科
- Publication Type:Journal Article
- Keywords:
CO poisoning;
Sulforaphane;
Mitochondrial ultrastructure;
Mitochondrial membrane potential;
Nrf-2;
Trx-1
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2018;25(4):213-219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of ultrastructural and functional impairment of mitochondria and the protective effect of sulforaphane (SFP) at different dosages on hippocampus neurons in rats after acute carbon monoxide(CO)poisoning.Methods One hundred and fifty adult healthy SD rats were randomly divided into the normal control group,the CO poisoning group and the SFP treatment group.The rat model of CO poisoning was established through exposure to CO in a hyperbaric chamber.The uhrastructural changes of mitochondria in nerve cells of the brain tissue were observed by transmission electron microscopy (TEM).Mitochondrial membrane potential (MFI) was detected with JC-1 fluorescent probe,and the expressions of Nrf-2 and Trx-1 proteins and mRNA were evaluated by immunohistochemistry and quantitative RT-PCR before and after intervention.Results CO poisoning could induce impairment of mitochondria ultrastructure in neurocytes in the brain tissue.SFP could obviously alleviate the damage of mitochondria ultrastructure.The MFI level in the brain tissue was obviously reduced in rats after CO exposure.Though MFI level was decreased 3 to 7 days after SFP administration,its level was significantly higher than that of the CO poisoning group at the same time point (P ＜0.05).As compared with those in the normal control group,the expression levels of Nrf-2 and Trx-1 proteins as well as their mRNA in the CO poisoning group were slightly increased (P ＜ 0.05).After treatment with SFP,the expressions of both the proteins and mRNA were obviously increased,and there was significant difference when compared with that of the CO poisoning group at the same time point (P ＜0.05).Conclusion Early administration of either moderate or high dose SFP could efficiently improve mitochondrial structure and function,enhance anti-oxidative stress of cells,thus exerting a positive effect against brain damage induced by acute CO poisoning.
