Effects of long non-coding RNA metastasis associated in lung denocarcinoma transcript 1 on high glucose-induced cardiomyocyte injury by regulating miR-320-3p-protein kinase B3 axis
10.3969/j.issn.1006-6187.2025.10.010
- VernacularTitle:长链非编码RNA人肺腺癌转移相关转录本1调节miR-320-3p-蛋白激酶B3轴对高糖诱导的心肌细胞损伤影响的研究
- Author:
Yongqiang WANG
1
;
Zongren WANG
;
Jingjing REN
Author Information
1. 453000 新乡市中心医院检验科
- Publication Type:Journal Article
- Keywords:
Metastasis associated in lung denocarcinoma transcript 1;
MiR-320-3p;
Protein kinase B3;
Cardiomyocyte
- From:
Chinese Journal of Diabetes
2025;33(10):780-788
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of long non-coding RNA(LncRNA)metastasis associated in lung denocarcinoma transcript 1(MALAT1)on high glucose-induced cardiomyocyte injury by regulating miR-320-3p-protein kinase B3(Akt3)axis.Methods H9c2 cells were divided into normal control(NC)group and high glucose(HG)group.After transfection,the cells were cultured in HG group were further divdided into subgroups as follows:HG+MALAT1 knock-down(HG+sh-MALAT1)group,HG+sh-NC group,HG+miR-320-3p mimic group,HG+mimic-NC group,HG+sh-MALAT1+miR-320-3p inhibitor group and HG+sh-MALAT1+pcDNA3.1-Akt3 group.Cell activity,apoptosis rate,B-cell lymphoma 2-related protein X(Bax),B-cell lymphoma factor 2(Bcl-2),cleaved caspase-3,Akt3 protein,MALAT1,miR-320-3p,Akt3 mRNA expression,interleukin(IL-1β),IL-6,tumor necrosis factor-α(TNF-α)were compared among the groups.Results The apoptosis rate of cardiomyocytes,the expressions of IL-1β,IL-6,TNF-α,Bax,cleaved caspase-3/caspase-3 protein,MALAT1 and Akt3 mRNA increased(P<0.05),while the expression of Bcl-2 protein and miR-320-3p decreased in HG group(P<0.05).After knocking down LncRNA MALAT1,apoptosis rate of HG-induced cardiomyocytes and levels of inflammatory factors were decreased(P<0.05).MALAT1 could negatively regulate expression of miR-320-3p,and miR-320-3p could negatively regulate expression of Akt3 protein.The overexpression of miR-320-3p could decrease apoptosis rate and inflammation level,while overexpression of Akt3 or inhibition of miR-320-3p expression could increase apoptosis rate of cardiomyocytes and inflammation level(P<0.05).Conclusions LncRNA MALAT1 can promote the expression of Akt3 protein by inhibiting miR-320-3p expression,thereby reduce activity of HG-induced cardiomyocytes and aggravate cardiomyocytes injury.
