Full genome analysis of G4P23porcine rotavirus and its pathogenicity in suckling mice and piglets
10.3969/j.issn.1002-2694.2025.00.142
- VernacularTitle:G4P[23]型猪轮状病毒的基因组序列分析及其对乳鼠和仔猪的致病性研究
- Author:
Hui DENG
1
;
Ran TAO
;
Nan HAN
;
Jianxin WANG
;
Xuefan SU
;
Chen WANG
;
Xi CHENG
;
Xianyu BIAN
;
Jiapeng SONG
;
Xuejiao ZHU
;
Xuehan ZHANG
;
Hongbo XIAO
;
Jinzhu ZHOU
;
Bin LI
Author Information
1. 湖南农业大学动物医学院,长沙 410128;江苏省农业科学院,兽医研究所/农业农村部兽用生物制品工程技术重点实验室,南京 210014
- Publication Type:Journal Article
- Keywords:
G4 genotype;
porcine rotavirus;
diarrhea;
suckling mice;
piglets;
pathogenicity
- From:
Chinese Journal of Zoonoses
2025;41(9):902-909
- CountryChina
- Language:Chinese
-
Abstract:
To perform the phylogenetic characterization of an isolated porcine rotavirus(PoRV)and investigate its pathogenicity in suckling mice and piglets.A G4P[23]genotype PoRV strain JSJR2023 was successfully isolated from the diarrheic piglet feces through propagation in MA104 cells.The viral proliferation kinetics were analyzed using TCID50 assays,followed by complete genome sequencing through Sanger sequencing platforms.Comprehensive genotyping and phylogenetic reconstruction were conducted using MEGA7.0 with maximum likelihood algorithms.Pathogenicity was assessed in the following animal models:5-day-old C57BL/6 mice and 3-day-old piglets.Multidimensional evaluation included clinical monitoring(diarrhea scoring,growth parameters),virological detection,and histopathological analysis of intestinal tissues.The virus strain JSJR2023 could replicate efficiently in MA104 cells,achieving peak titers of 107.5 TCID50/mL.Whole genome genotype analysis showed that the strain belonged to G4-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1.Phylogenetic analysis indicated that the VP3 and NSP4 genes of JSJR2023 strain were most closedrelated to human species rotaviruses,suggesting genetic reassortment between human and porcine RV strains.The animal experiments in suckling mice showed that the JSJR2023 strain infection caused diarrhea symptoms,intestinal edema and congestion,and shedding of intestinal villus epithelial cells.The pathogenicity experiments in piglets showed that compared with the control group,the challenged group of pig-lets had severe diarrhea symptoms,accompanied by reduced appetite and listlessness.Post-mortem examination revealed that the intes-tines were significantly thinner,congested,and filled with yellow watery contents.The challenged piglets showed typical pathological changes such as thinning of the intestinal wall and shortening and shedding of intestinal villi.In conclusion,this study successfully iso-lated a human-porcine recombinant G4P[23]PoRV strain and established the infection models in suckling mice and piglets,providing important tools for investigating the pathogenic mechanism of PoRV,evaluating vaccines and developing antiviral drug.
