Whole-genome molecular characterization analysis of a rotavirus vaccine-derived strain
10.3969/j.issn.1002-2694.2025.00.141
- VernacularTitle:一株轮状病毒疫苗衍生株的全基因组分子特征分析
- Author:
Xiaoping TANG
1
;
Yuhang WEI
;
Guangping XIONG
;
Xiao HU
;
Xiaoman SUN
;
Hong WANG
;
Jinsong LI
;
Lili LI
;
Ruyi CHE
;
Mengjie DONG
;
Wudi ZHANG
;
Zhaojun DUAN
;
Dandi LI
Author Information
1. 甘肃中医药大学公共卫生学院,兰州 730000;传染病溯源预警与智能决策全国重点实验室,国家卫生健康委员会医学病毒和病毒病重点实验室,中国疾病预防控制中心病毒病预防控制所,北京 102206
- Publication Type:Journal Article
- Keywords:
group A rotavirus;
G1P[8]genotype;
molecular characteristics
- From:
Chinese Journal of Zoonoses
2025;41(9):893-901
- CountryChina
- Language:Chinese
-
Abstract:
This study investigated the full-genome molecular characteristics of a rotavirus vaccine-derived strain,G1P[8]geno-type A group rotavirus RVA/Human-wt/CHN/HN1140/2021/G1P[8](referred to as HN1140).The gene fragments of the HN1140 strain were amplified with reverse transcription-polymerase chain reaction(RT-PCR)combined with whole-genome primers to obtain the full genome sequence.Genotyping was performed with the online genotyping tool RotaC 2.0,and similarity and genetic evolution analyses for each gene segment were conducted in DNAstar5.1 and MEGA11.0 software.The genotype of the HN1140 strain was deter-mined to be G1-P[8]-I2-R2-C2-M2-A3-N2-T6-E2-H3.Phylogenetic analysis demonstrated that all 11 genomic segments clus-tered closely with the RotaTeq vaccine strains,sharing 99.7%-100%nucleotide sequence similarity.Notably,VP1,VP2,VP6,and NSP2-NSP5 segments showed 100%nucleotide identity with RotaTeq strains.Comparative genomic analysis identified 13 nucleotide and 8 amino acid substitutions between HN1140 and RotaTeq strains,localized within the VP7,VP4,VP1,VP2,VP3,and NSP1 segments.The HN1140 strain exhibited the genotype G1-P[8]-A3-T6-H3,which was consistent with the typical profile of a vaccine-derived reassortant.This strain demonstrated high genetic similarity to RotaTeq vaccine strains,with nucleotide sequence identity ranging from 99.7%to 100%.These findings suggested that HN1140 evolved from RotaTeq vaccine strains through genetic reassortment.
