Whole genome characteristics of Salmonella from foodborne and diarrheal cases in Gansu Province from 2021 to 2023
10.3969/j.issn.1002-2694.2025.00.150
- VernacularTitle:2021-2023年甘肃省食源性和腹泻病例沙门菌全基因组特征研究
- Author:
Guang LAN
1
;
Yanqin SHEN
;
Jing ZHANG
;
Xinying LI
;
Jing YAN
;
Xiaoju LIU
;
Jie HE
;
Wei WANG
Author Information
1. 甘肃省疾病预防控制中心,兰州 730000
- Publication Type:Journal Article
- Keywords:
Salmonella;
serotype;
whole-genome sequencing;
antibiotic resistance gene;
virulence gene;
resistance gene
- From:
Chinese Journal of Zoonoses
2025;41(9):952-959
- CountryChina
- Language:Chinese
-
Abstract:
Salmonella,an important foodborne pathogen,is responsible for numerous diseases in both humans and animals.We conducted a genome-wide analysis of Salmonella isolates from diarrheal and foodborne infection cases in Gansu Province between 2021 and 2023.A total of 163 Salmonella strains were collected and subjected to biochemical identification,followed by serological typing,whole-genome sequencing,and bioinformatics characterization.The results revealed 27 distinct serotypes,among which Sal-monella typhimurium variant(S.4,[5],12∶1∶-),Salmonella enteritidis,and Salmonella enterica London were predominant.Notably,the serotype distribution exhibited significant variation across sample sources.Multilocus sequence typing(MLST)classified the iso-lates into 27 sequence types(STs),among which ST34,ST11,ST155,and ST19 had the highest prevalence.The MLST profiles dem-onstrated strong concordance with serological classifications.For Salmonella,we detected a total of 17 673 virulence genes in 374 cat-egories,carrying multiple virulence islands.Some strains carried virulence plasmid genes,among which 45 strains of Salmonella enter-itidis had higher types and numbers of virulence factors detected than other serotypes of Salmonella.Antimicrobial resistance profiling identified 69 resistance genes conferring resistance to 13 classes of antimicrobial agents,and multidrug resistance patterns were preva-lent among isolates.Plasmid characterization revealed 35 plasmid types,some containing antimicrobial resistance determinants.Addi-tionally,three disinfectant resistance genes were identified.This study highlights the extensive diversity of Salmonella serotypes and STs in Gansu Province and the complex repertoire of virulence and resistance genes.The emergence of disinfectant resistance genes un-derscores challenges in conventional disinfection protocols.These findings provide critical insights for refining Salmonella surveillance and control strategies in public health and food safety contexts.
