Determination of 10 drugs including morphine in hair using triple quadrupole tandem mass spectrometry
10.13618/j.issn.1001-5728.2025.03.012
- VernacularTitle:基于三重四极杆串联质谱检测毛发中吗啡等10种毒品
- Author:
Meiting LIN
1
;
Xueyan ZHU
;
Jian LI
;
Quanlu DOU
;
Xin WANG
;
Ping XIANG
;
Junbo ZHAO
Author Information
1. 司法鉴定科学研究院,上海市法医学重点实验室,司法部司法鉴定重点实验室,上海市司法鉴定专业技术服务平台,上海 200063;沈阳药科大学药学院,辽宁沈阳 116023
- Publication Type:Journal Article
- Keywords:
Hair;
Drug;
Triple quadrupole tandem mass spectrometry;
Scheduled MRM mode
- From:
Chinese Journal of Forensic Medicine
2025;40(3):330-337
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a sensitive,accurate and rapid detection method for 10 drugs and metabolites in hair with triple quadrupole tandem mass spectrometry,addressing the identification of drugs in real hair samples.Methods After cryogenic grinding and ultrasonic extraction,hair was separated by Restek Allure PFPP column(100 mm × 2.1 mm,5 μm).The mobile phase A was 20 mmol/L ammonium acetate,0.1％formic acid and 5％acetonitrile aqueous solution.The mobile phase B was acetonitrile.An electrospray ionization source was used for data acquisition in scheduled MRM mode.Results The method showed good linearity for all analytes within the validated range(R2＞0.995),with the limits of detection ranging from 0.5 to 6 pg/mg,the limits of quantification ranging from 2.5 to 10 pg/mg.Accuracy ranged from 89.1％to 114.6％,with intra-day precision ranging from 0.2％to 11.7％,inter-day precision ranging from 4.0％to 15.8％.the matrix effects ranging from 89.4％to 118.4％,the recoveries ranging from 63.6％～112.1％.Totally 20 cases were detected positive in 196 actual hair samples.Conclusion The time-scheduled scanning method exhibits high stability and sensitivity,enabling high-throughput detection,and is suitable for forensic toxicology laboratories to identify drugs in hair.
