Chiral analysis of dextromethorphan and levomethorphan in human hair and urine
10.13618/j.issn.1001-5728.2025.03.001
- VernacularTitle:头发和尿液中右美沙芬和左美沙芬的手性分析
- Author:
Qiao YANG
1
;
Jian ZHANG
;
Rui HU
;
Bingbing TIAN
;
Zhonghui ZHANG
Author Information
1. 南京市公安局禁毒支队,江苏南京 210042
- Publication Type:Journal Article
- Keywords:
Dextromethorphan;
Levomethorphan;
Chiral;
Hair;
Urine
- From:
Chinese Journal of Forensic Medicine
2025;40(3):267-272
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for chiral analysis of dextromethorphan and levomethorphan in hair and urine by high performance liquid chromatography-triple quadrupole mass spectrometry.Methods Hair samples were extracted by grinding ultrasound,and urine samples were extracted by acetonitrile.The supernatant were separated using a Astec CHIROBIOTIC? V2 column(25 cm × 4.6 mm,5 μm)with 0.5 ‰ formic acid and 10 mmol/L ammonium acetate-methanol as mobile phases,then analyzed in scheduled-multiple reaction monitoring mode.Results The limits of detection and lower limits of quantitation of dextromethorphan and levomethorphan were 0.001 ng/mg and 0.005 ng/mg in hair samples,while they were 0.050 ng/mL and 0.200 ng/mL in urine samples.In the corresponding concentration range,there were good linearity for chiral isomers with correlation coefficients over 0.999.Besides,the recovery rates,accuracy and precision met analytical requirements,and no significant matrix effect were exhibited.In the actual case samples,dextromethorphan were detected in hair of 12 cases and in the corresponding urine of 3 cases.Conclusion This method is rapid,sensitive and reliable for chiral analysis of dextromethorphan and levomethorphan in hair and urine.
