Transient Expression of Monkeypox Virus Recombinant Protein B6R-Fer in Nicotiana benthamiana
10.13865/j.cnki.cjbmb.2025.07.1171
- VernacularTitle:猴痘病毒重组蛋白B6R-Fer在本氏烟草中的瞬时表达
- Author:
Ya-Hui WU
1
;
Yan-Ting QI
;
Yu-Han WANG
;
Wei-Song PAN
;
Jian QIU
;
Chuan WU
Author Information
1. 中南大学冶金与环境学院,长沙 410083
- Publication Type:Journal Article
- Keywords:
monkeypox virus(MPXV);
recombinant protein;
plant bioreactor;
transient expression
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(9):1342-1348
- CountryChina
- Language:Chinese
-
Abstract:
Monkeypox is a viral zoonotic disease,and there is currently a lack of safe and effective vac-cines against the monkeypox virus.Therefore,screening and developing vaccine candidates is of signifi-cant practical importance.With the rapid advancement of molecular biology and plant genetic engineer-ing,plant bioreactors offer promising potential for producing vaccine proteins due to their advantages,in-cluding safety,cost-effectiveness,and scalability.In this study,we focused on the monkeypox protein B6R.The recombinant expression plasmid pFolia40108-B6R-Fer was successfully constructed using am-plification,enzyme digestion,and flexible linker tandem ferritin technology.A complete transient expres-sion system in Nicotiana benthamiana and a purification system for the recombinant monkeypox protein were established.The optimal expression time was determined to be 12-14 days,with a final purified pro-tein concentration of approximately 1 mg/mL and a yield of 0.85 mg/kg fresh weight.The purified B6R-Fer recombinant protein self-assembled into spherical virus-like particles(VLPs)with an average particle size of 24 nm.The B6R-Fer recombinant protein from this study shows promising potential for use in the development and screening of plant-derived monkeypox vaccine candidates.
