Simultaneous determination of ephedrine and pseudoephedrine in human urine using gas chromatography-tandem mass spectrometry
10.13618/j.issn.1001-5728.2025.03.013
- VernacularTitle:GC-MS/MS法同时测定人尿液中麻黄碱和伪麻黄碱
- Author:
Yuxuan CHEN
1
;
Huimin ZHANG
;
Xiaolong ZHANG
;
Mengchao WANG
;
Kundi ZHAO
;
Yinyin DAI
;
Jie GU
;
Wurita AMIN
;
Liqin CHEN
Author Information
1. 内蒙古医科大学研究生学院,内蒙古呼和浩特 010059
- Publication Type:Journal Article
- Keywords:
Forensic toxicology;
Urine;
GC-MS/MS;
Ephedrine;
Pseudoephedrine
- From:
Chinese Journal of Forensic Medicine
2025;40(3):338-342,347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a gas chromatography-tandem mass spectrometry(GC-MS/MS)method for the simultaneous determination of ephedrine and pseudoephedrine in urine.Methods Urine samples containing ephedrine and pseudoephedrine components were extracted with ethyl acetate,centrifuged to collect the supernatant and evaporated to dryness under a nitrogen stream and then derivatized with heptafluorobutyric anhydride 60 μL at 70 ℃ for 30 min,and re-evaporated under nitrogen,and then solubilized with 50 μL of methanol,and then analyzed by GC-MS/MS.Results The method demonstraed excellent linearity for ephedrine(0.05～10 μg/mL,r=0.999 8)and pseudoephedrine(0.02～5 μg/mL,r=0.999 5).Extraction recoveries ranged from 89.4％～95.8％(ephedrine)and 90.3％～93.8％(pseudoephedrine).Limits of detection and quantification of ephedrine and pseudoephedrine were 0.005 μg/mL and 0.01 μg/mL,the intra-day precision and accuracy were less than 5.87％and 9.56％,respectively,and the inter-day precision and accuracy were less than 7.54％and 9.27％,respectively.The stability of ephedrine and pseudoephedrine in urine in 15 d was good under the conditions of room temperature and-20 ℃.Conclusion The GC-MS/MS analytical method for the analysis of ephedrine and pseudoephedrine components in urine established in this study is accurate,stable and sensitive,which can provide data technical support for the forensic toxicological analysis of amphetamine-type drugs or new psychoactive substances in the cathinone group.
