Analysis of Dabie bandavirus infection and genetic characteristics in a city of Hebei Province
10.3969/j.issn.1002-2694.2025.00.048
- VernacularTitle:河北省某市大别班达病毒感染情况及病毒遗传特征分析
- Author:
Luling LI
1
;
Yamei WEI
;
Minghao GENG
;
Yan ZHENG
;
Yanan CAI
;
Xu HAN
;
Zhenkun WANG
;
Zhengguang WANG
;
Qi LI
Author Information
1. 河北医科大学公共卫生学院,石家庄 050017;河北省疾病预防控制中心,石家庄 050021
- Publication Type:Journal Article
- Keywords:
Dabie bandavirus;
severe fever with thrombocytopenia syndrome;
evolutionary analysis;
tick
- From:
Chinese Journal of Zoonoses
2025;41(8):801-808
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at investigating the prevalence of Dabie bandavirus(DBV)infection in suspected cases of se-vere fever with thrombocytopenia syndrome(SFTS)and tick specimens in Cangzhou City from 2023 to 2024,and providing scientific basis for the prevention,control and pathogen surveillance of SFTS in this area.Pathogen detection and virus isolation were performed on suspected SFTS cases and tick specimens collected in Cangzhou City from 2023 to 2024.The next-generation sequencing technol-ogy was used to conduct whole-genome sequencing of DBV.A phylogenetic tree was constructed using software such as MEGA,DNA Star,and homology comparison and amino acid variation site analysis were carried out.Among the 70 suspected SFTS cases,the DBV positive detection rate was 60%(42/70).The positive cases were mainly farmers aged 50-79,concentrated in Yanshan County and Haixing County.402 ticks were pooled into 31 sample tubes,among which 2 tubes tested positive for DBV nucleic acid,with a mini-mum infection rate of 0.50%.A total of 9 human-derived and 2 tick-derived DBV strains were isolated.The whole-genome sequencing results showed that all strains clustered within the F branch,with sequences homology exceeding 99%.There were a total of 10 amino acid variation sites in the RdRP,GP and NSs sequences.Therefore,the distribution of SFTS cases in Cangzhou City exhibited regional clustering.The overall level of DBV infection in ticks in this region was relatively low.It is recommended to further improve the disease early warning mechanism and continue to conduct pathogen surveillance.
