LncRNA SNHG12 Promotes Breast Cancer Progression via Competing with EPHB3 for Binding to miR-326
10.13865/j.cnki.cjbmb.2025.08.1241
- VernacularTitle:LncRNA SNHG12与EPHB3竞争性结合miR-326促进乳腺癌的进展
- Author:
Yong LI
1
;
Yi-Ning QUAN
;
Kun WANG
;
Hong-Li LI
;
Chong-Gao YIN
Author Information
1. 山东第二医科大学医学研究实验中心,山东 潍坊 261053
- Publication Type:Journal Article
- Keywords:
breast cancer(BRCA);
long noncoding RNA SNHG12(LncRNA SNHG12);
miR-326;
EPH receptor B3(EPHB3);
competing endogenous RNA(ceRNA);
invasion;
migration
- From:
Chinese Journal of Biochemistry and Molecular Biology
2025;41(9):1310-1319
- CountryChina
- Language:Chinese
-
Abstract:
Breast cancer(BRCA)remains one of the leading causes of cancer-related deaths worldwide due to its high rates of metastasis and recurrence,making it crucial to explore its underlying molecular mechanisms.Our previous study demonstrated that miR-326 inhibits BRCA progression by targeting EPH receptor B3(EPHB3).This study further explores the molecular mechanism by which long non-coding RNAs(LncRNAs)regulates BRCA progression via the competing endogenous RNA(ceRNA)mecha-nism,in which it competes with EPHB3 for miR-326 binding.Bioinformatics analysis identified LncRNA Small Nucleolar RNA Host Gene 12(SNHG12)as a potential miR-326-binding molecule.SNHG12 was found to be significantly upregulated in BRCA tissues,exhibiting a negative correlation trend with miR-326 and a positive correlation trend with EPHB3,suggesting its potential involvement in the ceRNA regu-latory network.Nuclear-cytoplasmic fractionation assays revealed cytoplasmic localization of SNHG12,while dual-luciferase reporter assays confirmed its direct binding to miR-326.Functional experiments demonstrated that SNHG12 knockdown significantly suppressed BRCA cell proliferation,invasion,and migration,while miR-326 inhibition reversed these effects.Furthermore,miRNA pulldown assay re-vealed significant enrichment of SNHG12 and EPHB3 in the miR-326 pulldown products,indicating di-rect binding between them.Western blotting and rescue experiments revealed that SNHG12 upregulates EPHB3 expression by sponging miR-326,thereby promoting the malignant behaviors of BRCA cells.Col-lectively,this study revealed that LncRNA SNHG12 promotes BRCA progression by regulating the miR-326/EPHB3 axis through a ceRNA mechanism.The SNHG12/miR-326/EPHB3 pathway may represent a promising target for the molecular diagnosis and targeted therapy of BRCA.
