Preparation and initial identification of monoclonal antibody of anti-vibrio fluvialis
10.3760/cma.j.issn.1009-6906.2012.04.002
- VernacularTitle:抗河流弧菌单克隆抗体的建立及初步鉴定
- Author:
Kai-fei FU
1
;
Cong MA
1
;
Jian-wei GUO
1
;
Xiu-hong HAO
1
Author Information
1. 海军总医院中心实验科,北京,100048
- Publication Type:Journal Article
- Keywords:
Vibrio fluvialis;
Monoclonal antibody;
ELISA
- From:
Chinese journal of nautical medicine and hyperbaric medicine
2012;19(4):198-201
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare high-performance and specific monoclonal antibody of vibrio fluvialis,and to provide a technical platform for the development of a rapid ELISA kit.Methods BALB/c mice (8 weeks) were immunized with inactivated vibrio fluvialis,and hybridoma cell strains of anti-vibrio fluvialis were prepared with cell-fusion technology.Chromatosomes of hybridoma cells were identified with Giemsa staining,and the potency and cross-reactivity of anti-vibrio fluvialis as well as other important marine bacteria were screened and identified with ELISA.Results Four strains of hybridoma (named as 01H10,02F12,05F3 and 03G10 ) were obtained,all having the features of hybridoma cell chromatosomes,and with good specificity and immunorcactivity.The hybridoma also had favourable specificity and immunoreactivity.Conclusions Specific antibodies of anti-vibriofluvialis prepared and screened in the study would be useful for the development of the rapid detection kit of vibrio fluvialis.
