Implication of histamine H1 receptors in vestibular nucleus and motion sickness
10.3760/cma.j.issn.1007-6239.2011.03.006
- VernacularTitle:前庭核H1受体在运动病发生中的作用
- Author:
Li-gui HUANG
1
;
En-tong WANG
;
Wei CHEN
;
Wei-xi GONG
Author Information
1. 解放军庐山疗养院耳鼻咽喉头颈外科
- Publication Type:Journal Article
- Keywords:
Motion sickness;
Histamine;
Receptors,histamine H1;
Promethazine;
Rats
- From:
Chinese Journal of Aerospace Medicine
2011;22(3):189-193
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of histamine H1 receptors (H1 R) in vestibular nucleus of rat' s brainstem and the underlying role of H1R in motion sickness (MS).Methods Total of 24 healthy Sprague-Dawley rats were randomly divided into four groups,6 rats in each group.A:MS(-)/Drug(-) control group,in which the rats were not exposed to motion stimulus and no anti-motion sickness drug promethazine taking； B:MS(+)/Drug(-) group,only exposed to motion stimulus; C:MS(-)/Drug(+) group,that applied with promethazine but not exposed to motion stimulus； D:MS(+)/Drug (+) group,applied with promethazine (0.25 mg for each rat) 30 minutes before exposing to motion stimulus.MS was induced by a complex motion stimulus and the conditioned taste aversion was used as the behavioral indicator of MS in rats,measuring the intaked volume of 0.15％ sodium saccharin solution (SSS) for each rat 45 minutes after motion stimulus.H1 R in the vestibular nucleus of brainstem was examined by immunofluorescence staining.The expression of H1 R proteins in hrainstem tissue at vestibular nucleus level was detected by Western blot.The effects of motion stimulus and anti-MS drug promethazine on the expressions of H1R were evaluated.Results The mean intake of SSS in B group was significantly less than that in A group (F=346.82,P＜0.01),which demondtrated that MS was induced by this motion stimulus in the rats.The mean intaked SSS volume (14.8 ml) in C group was similar to that in A group (P＞0.05),indicating no significant effect of promethazine on intaking SSS.The mean intaked SSS volume in D group was more than that in B group (P＜0.01),but less than that in A group or C group (P＜0.01),which indicated that the inhibited intaking of SSS in motion-stimulated rats was improved partially by promethazine.Immunofluorescence staining showed the positive expression of H1R in the vestibular nucleus of brainstem in rats and the expression was enhanced by motion stimulus,but was not significantly affected by promethazine.Western blot analysis showed that H1 R proteins expressed in the brainstem tissues at vestibular nucleus level,which were also increased significantly after motion stimulus,but the increase was not affected by promethazine.Conclusions H1 R exists in vestibular nucleus of brainstem in rats.The expression of H1R is up-regulated by motion stimulus,but is not affected by anti-MS drug promethazine.It indicates that histaminergic system is involved in the development of MS.Promethazine,as a blocker H1R,plays the role of anti-MS by binding H1R rather than by increasing the expression of H1R.
