Establishment of indirect competitive ELISA method for detection of ribavirin in chicken
10.3969/j.issn.1000-484X.2025.10.031
- VernacularTitle:鸡肉中利巴韦林间接竞争ELISA检测方法的建立
- Author:
Xiaofei HU
1
;
Yunrui XING
1
;
Guangxu XING
1
;
Yaning SUN
1
;
Lin WANG
1
;
Gaiping ZHANG
1
Author Information
1. 河南省农业科学院动物疫病防控研究所(河南省农业科学院动物免疫学重点实验室),郑州 450002
- Publication Type:Journal Article
- Keywords:
Ribavirin;
Antiviral drugs;
Chicken;
Detection;
icELISA
- From:
Chinese Journal of Immunology
2025;41(10):2495-2498,2504
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a highly sensitive indirect competitive ELISA(icELISA)method for detecting ribavirin in chicken.Methods:Based on the obtained monoclonal antibodies against ribavirin,a chessboard test was employed to determine the optimal working concentration of artificial antigen and antibody,and then established an icELISA method.Furthermore,performance of the detection method was evaluated.Results:The established icELISA method has a linear range of 0.44～32.71 ng/ml,IC50 of which was 3.78 ng/ml,and the limit of detection(LOD)was 0.20 ng/ml.Except for specific reaction with ribavirin,there were no cross reactions with other antiviral drugs.Recovery rate of sample spiking was between 91.60%and 100.76%,and coefficient of variation was between 7.29%and 10.63%.Conclusion:A highly sensitive and specific icELISA method for detection of ribavirin has been estab-lished,which can be used to determine the residue of ribavirin in chicken.
