Allergens elicit elevated expressions of IL-18 and IL-18Rα in blood CD4+Th17 cells of patients with allergic rhinitis and asthma syndrome
10.3969/j.issn.1000-484X.2025.10.027
- VernacularTitle:过敏原诱导过敏性鼻炎合并哮喘综合征患者外周血CD4+Th17细胞表达IL-18和IL-18Rα的研究
- Author:
Junling WANG
1
;
Mengmeng ZHAN
;
Zhaolong ZHANG
;
Shaoheng HE
;
Bingyu QIN
Author Information
1. 郑州大学人民医院河南省人民医院重症医学部,郑州 450003;锦州医科大学附属第一医院变态反应与临床免疫研究中心,锦州 121001
- Publication Type:Journal Article
- Keywords:
Allergic rhinitis and asthma syndrome;
Th17 cell;
IL-18;
IL-18Rα;
Allergen
- From:
Chinese Journal of Immunology
2025;41(10):2469-2474
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of allergens on expressions of IL-18,IL-18BPa and IL-18Rα in blood CD4+Th17 cells of patients with allergic rhinitis and asthma syndrome(ARA)and plasma levels of IL-17A and IL-17F in ARA patients.Methods:ARA patients(n=25)and healthy volunteers(n=22)in the First Affiliated Hospital of Jinzhou Medical University and Henan Provincial People's Hospital were recruited,and blood samples of the subjects were collected.Proportions of CD4+Th17 cells,and effects of allergens on expressions of IL-18,IL-18BPa and IL-18Rα in Th17 cells were determined by flow cytometry.Levels of IL-17A and IL-17F were examined by Bioplex system.Correlation between levels of free plasma free IL-18(fIL-18)and IL-17A,and per-centage of Th17 cells was further analyzed.Results:Proportion of IL-18+cells in Th17 cells was increased in ARA patients by 26.0%(P<0.01),and house dust mite allergen induced 1.22-fold elevation in expression of IL-18 in Th17 cells(P<0.05).In addition,Arte-misiae sieversiana wild pollen allergen enhanced expressions of IL-18 and IL-18Rα in Th17 cells of ARA patients(P<0.05).Plasma levels of IL-17A and IL-17F were increased by 2.2 and 1.1 folds,respectively(P<0.05)in ARA patients,and they correlated well with other(R=0.712,P<0.01).Moreover,the increased level of fIL-18 was moderately correlated with the increased level of IL-17A and the elevated proportion of Th17 cells in ARA patients(r=0.607,r=0.652,P<0.05).Conclusion:The increased plasma fIL-18 in ARA patients may be derived from Th17 cells.Allergens may be involved in pathogenesis of ARA by inducing elevated IL-18 and IL-18Rα expressions in Th17 cells.
