Chemokine ligand 17 promotes proliferation of cervical cancer cells through interleukin-27 mediated polarization of M2 macrophages
10.3969/j.issn.1000-484X.2025.10.021
- VernacularTitle:趋化因子配体17通过IL-27介导的M2巨噬细胞极化促进宫颈癌细胞的增殖
- Author:
Hangcheng LU
1
;
Zhenzhen LAI
;
Chengcai KONG
;
Yishan DONG
Author Information
1. 南京医科大学常州医学中心常州市妇幼保健院,常州 213000
- Publication Type:Journal Article
- Keywords:
Cervical cancer;
Macrophages;
Tumor microenvironment;
CCL17
- From:
Chinese Journal of Immunology
2025;41(10):2427-2434
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether the highly expressed chemokine ligand 17(CCL17)in cervical cancer(CC)pro-motes the progression of CC through M2 macrophage polarization.Methods:The effect of CCL17 on the expression of IL-27 in CC cell line was detected by flow cytometry and ELISA.The effects of CCL17 and IL-27 on proliferation and differentiation of U937 cells were analyzed.The co-culture model of macrophages and CC cells were established to detect the effects of CCL17 on the proliferation of U937 and CC cells.Results:The CC cells secreted significantly higher levels of IL-27 after stimulation with CCL17.In addition,IL-27 but not CCL17 significantly upregulated interleukin 27 receptor(IL-27R,consisted of WSX-1 and GP130)on U937 cells.IL-27 de-rived from CC cells can promote the proliferation of U937 cells and induce the polarization of M2 macrophages.In the co-culture sys-tem,the level of IL-27 secreted by CC cells stimulated by CCL17 was significantly increased,and both CCL17 and IL-27 promoted the proliferation of CC and U937 cells,and the effect of CCL17 was partially reversed by αIL-27.Conclusion:CCL17 promotes the proliferation of macrophages and induces M2 polarization by stimulating CC cells to secrete IL-27,thereby enhancing the crosstalk be-tween CC cells and macrophages.Eventually,CCL17 participates in the malignant evolution of CC.
