Effects of intrauterine perfusion of dexamethasone on endometrial fibrosis and inflammation after intrauterine adhesion decomposition
10.3969/j.issn.1000-484X.2025.10.004
- VernacularTitle:宫腔粘连分解术后宫腔灌注地塞米松对子宫内膜纤维化及炎症的影响
- Author:
Jianhua FU
1
;
Mengxue WU
1
;
Xiuying LIN
1
;
Xuguang MI
1
;
Xue WANG
1
;
Huanhuan ZANG
1
;
Wenqi ZHANG
1
;
Yanqiu FANG
1
Author Information
1. 吉林省人民医院生殖医学中心,长春 130000
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Endometrial fibrosis;
Intrauterine perfusion;
TGF-β1;
Endometrial receptivity
- From:
Chinese Journal of Immunology
2025;41(10):2324-2328
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate improvement of dexamethasone(DM)treatment after intrauterine adhesion decomposition on uterine fibrosis and inflammatory factors.Methods:A total of 60 patients with moderate to severe intrauterine adhesions admitted to The Reproductive Medicine Center of Jilin Provincial People's Hospital from March 2021 to March 2023 were selected,and divided into experimental group and control group according to random number table method,with 30 cases in each group.Control group under-went simple hysteroscopic adhesion decomposition,and experimental group underwent intrauterine infusion treatment with DM injec-tion after intrauterine adhesion decomposition.Improvement of clinical symptoms in the two groups was observed.RT-qPCR was used to detect changes in mRNA levels of intimal receptivity-related factors ER,EGFR,LIF,ITGB3,HOXA10,HOXA11,fibrosis-related factors TGF-β1,E-cadherin,N-cadherin,Smad,and inflammatory related factors IL-8,IL-2 and CD138 in two groups.Western blot was used to detect changes in expression levels of inflammatory related proteins IL-8,IL-2 and CD138.Results:Compared with before treatment,endometrial thickness,mRNA levels of ER,EGFR,LIF,ITGB3,HOXA10,HOXA11 and E-cadherin were significantly increased,while mRNA levels of TGF-β1,N-cadherin,Smad,IL-8,IL-2 and CD138,protein levels of IL-8,IL-2 and CD138,and endometrial blood flow parameters PI,RI and adhesion were significantly decreased.Eendometrial thickness,mRNA levels of ER,EGFR,LIF,ITGB3,HOXA10,HOXA11 and E-cadherin in experimental group were significantly higher than those in control group,while mRNA levels of IL-8,IL-2,TGF-β1,N-cadherin,Smad,CD138,IL-8,IL-2 and CD138,and endometrial blood flow parameters PI,RI and adhesion were lower than those in control group(P<0.05).Conclusion:Treatment of DM after intrauterine adhe-sion decomposition can improve intrauterine adhesion,blood flow parameters and endometrial receptivity,and prevent the recurrence of intrauterine adhesion,which mechanism may be related to up-regulation of E-cadherin expression and down-regulation of IL-8,IL-2,CD138,TGF-β1,N-cadherin and Smad expressions.
