The effect of Nel-like type 1 molecule on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth
10.13591/j.cnki.kqyx.2025.07.007
- VernacularTitle:神经表皮生长因子样蛋白1对人脱落乳牙牙髓干细胞增殖和骨向分化的影响
- Author:
Chen YU
1
;
Longfei HE
;
Yao FENG
;
Xiao GE
;
Yunting CUI
;
Ti LI
;
Miao YU
Author Information
1. 山东第二医科大学口腔医学院,山东潍坊(261000)
- Publication Type:Journal Article
- Keywords:
Nel-like type 1 molecule;
stem cells from human exfoliated deciduous teeth;
osteogenic differentiation
- From:
STOMATOLOGY
2025;45(7):518-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Nel-like type 1 molecule(NELL-1)on the proliferation and osteogenic differenti-ation of stem cells from human exfoliated deciduous teeth(SHEDs).Methods SHEDs was isolated,cultured,and identified.The third generation SHEDs were used for subsequent experiments.SHEDs were divided into 4 groups,and NELL-1 was added to each group at concentrations of 0(control group),50,100,and 200 ng/mL.Cell activity was measured by CCK-8 assay and crystal violet staining.The changes in osteogenic ability were detected by alkaline phosphatase activity.The expression levels of osteogenesis-related genes ALP,OCN and Runx-2 by RT-qPCR were detected.Western blot was used to detect the expression of osteogenesis-related pro-teins ALP,Runx-2 and Col-Ⅰ.Results SHEDs exhibited stem cell characteristics,and 50 ng/mL of NELL-1 protein had a promo-ting effect on SHEDs proliferation(P＜0.01).Alkaline phosphatase activity assay showed that after the addition of NELL-1,the osteo-genic effect of each group was better than that of the control group and 50 ng/mL of NELL-1 was the best(P＜0.05).RT-qPCR and Western blot results showed that 50 ng/mL of NELL-1 significantly promoted the expression of osteoblast-related genes including ALP,OCN and Runx-2 and the protein expression of ALP,Runx-2 and Col-Ⅰ(P＜0.05).Conclusion NELL-1 can promote the prolifera-tion and osteogenic differentiation of SHEDs.
