The role of advanced oxidation protein products in epithelial mesenchymal transition of small intestinal epithelial cells
10.3760/cma.j.cn101480-20200217-00015
- VernacularTitle:晚期氧化蛋白产物在小肠上皮细胞的上皮间质转化中的作用
- Author:
Yu ZHENG
1
;
Zongqiang NIE
1
;
Hongyuan CHEN
1
;
Xiangyu WANG
1
;
Haixiao HUANG
1
;
Liangxiang HUANG
1
;
Changqing ZENG
1
Author Information
1. 福建医科大学省立临床医学院 福建省立医院胃肠外科，福州 350001
- Publication Type:Journal Article
- Keywords:
Advanced oxidation protein products;
Epithelial-mesenchymal transition;
Small intestinal epithelial cells;
Intestinal fibrosis;
Inflammatory bowel disease
- From:
Chinese Journal of Inflammatory Bowel Diseases
2020;04(2):124-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of advanced oxidation protein products (AOPPs) in epithelial - mesenchymal transition (EMT) of small intestinal epithelial cells, and provide basis for the study of mechanism of intestinal fibrosis (IF) in intestinal bowel disease (IBD) .Methods:The small intestinal crypt epithelial cells IEC-6 were cultured in vitro. AOPPs was made by hypochlorous acid oxidation of rat serum albumin (RSA) in vitro. IEC-6 cells were divided into blank control group, AOPPs group and RSA group. The different concentrations (50, 100, 200, 400 μg/ml) of AOPPs were used to interfere with IEC-6 cells respectively in AOPPs group, 50, 100, 200, 400 μg/ml RSA were used to interfere with IEC-6 cells respectively in RSA group and no intervention in blank control group. The morphological changes of IEC-6 cells were observed and apoptosis was detected by flow cytometry in 3 groups 24 h after intervention. When the apoptosis rate was highest, the concentration of AOPPs were analyzed statistically and used to interfere IEC-6 for 48 and 72 h. The difference of apoptosis rate were analyzed between the different times. The mRNA expression of E-cadherin, Fibronectin, Snail, Slug and Collagen Ⅰ were detected in blank control group, AOPPs group and RSA group by fluorescence quantitative PCR.Results:AOPPs could induce the apoptosis of IEC-6 cells with time- and concentration-dependent effects ( P<0.05) . The apoptosis rate was (17.30 ± 1.03) %, which reached the peak by the intervention of 400 μg/ml AOPPs for 72 h. AOPPs significantly decreased the mRNA expression of E-cadherin in IEC-6 cells, significantly increased the mRNA expression of Fibronectin and CollagenⅠ, and significantly increased the mRNA expression of transcription factor Snail and Slug (all P<0.05) . Conclusions:AOPPs can promote the ECT of small intestinal epithelial cells and play a role in IF of IBD by inducing the apoptosis of IEC-6 cells, inhibiting of the transcription of E-cadherin, up-regulating the gene expression of the transcription factors Snail and Slug, while increasing the gene expression of Fibronectin and CollagenⅠ.
