Bioinformatics analysis of exercise rehabilitation in improving the non-alcoholic fatty liver fibrosis
10.3969/j.issn.1001-1242.2025.07.003
- VernacularTitle:运动改善小鼠非酒精性脂肪肝纤维化的生物信息学分析
- Author:
Jia-hao XU
1
;
Jiling LIANG
;
Jiahang LI
Author Information
1. 武汉体育学院运动医学院,湖北省 武汉市,430079
- Publication Type:Journal Article
- Keywords:
liver fibrosis;
exercise;
rehabilitation;
high-intensity intermittent exercise;
bioinformatics
- From:
Chinese Journal of Rehabilitation Medicine
2025;40(7):986-994
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Hepatic fibrosis is usually the result of chronic liver disease damage,which may develop into cir-rhosis and liver cancer.Exercise can improve the progression of liver fibrosis,but its related mechanisms are still unclear.This study combined transcriptome,gene ontology(GO),Kyoto Encyclopedia of Genes and Ge-nomes(KEGG)analysis,Cytoscape,and other bioinformatics technologies to screen key genes and related pathways in the exercise intervention regulating liver fibrosis.Method:The liver of nonalcoholic fatty liver disease(NAFLD)mice fed a high-fat diet was sequenced by transcriptome,and the differentially expressed genes(DEGs)between the model group and different exercise groups(aerobic exercise group and high-intensity intermittent exercise group)were selected for intersection.The screening conditions were qValue<0.05 and|FoldChange|>2,and the differentially expressed genes(DEGs)were determined.To further determine the hub genes and key enrichment pathways involved in motor regula-tion of fibrosis.Result:Compared with the model group,the aerobic exercise group(Group A)had 144 upregulated genes and 930 downregulated genes.The high-intensity intermittent exercise group(H group)had 122 upregulated genes,559 downregulated genes,and 23 Hub genes.GO and KEGG results showed that the functions and pathways of DEG enrichment included cell adhesion,collagen fiber tissue,protein binding,ECM receptor in-teraction,and the PI3K/Akt signaling pathway,and 23 key genes were screened.Conclusion:Exercise can promote the rehabilitation of nonalcoholic fatty liver-related liver fibrosis.The key pathway and Hub genes are screened through bioinformatics,which is expected to be biomarkers in exercise-re-lated nondrug rehabilitation strategies for liver fibrosis.
