Establishment and Validation of Prognostic Model for Aging-related Genes of Squamous Cell Lung Cancer Based on TCGA Database
10.13241/j.cnki.pmb.2025.17.002
- VernacularTitle:基于TCGA数据库肺鳞癌衰老相关基因预后模型的建立与验证
- Author:
Yu-lin HE
1
;
Shao-zhang ZHOU
;
Qi-chang HUANG
;
Tian GUO
Author Information
1. 广西医科大学肿瘤医学院 广西南宁 530021
- Publication Type:Journal Article
- Keywords:
Lung squamous cell carcinoma(LUSC);
Aging-related genes(ARGs);
Prognostic model;
Bioinformatics analysis;
Immune cell infiltration
- From:
Progress in Modern Biomedicine
2025;25(17):2729-2739
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of aging-related genes(ARGs)in the prognosis of lung squamous cell carcinoma(LUSC)and establish a novel prognostic prediction model.Methods:Transcriptomic data and clinical information of LUSC patients were obtained from TCGA,combined with ARGs from Aging Atlas.Key genes were screened through differential expression analysis,survival analysis,and Cox regression to construct a prognostic model.Model performance was validated in clinical subgroups,and biological pathway enrichment(GSEA)and immune microenvironment analyses were performed.Results:Five ARGs(ERFFI1,MDH1,SENP2,SNAI1,TP63)were identified to build the model.Significant survival differences were observed between high-and low-risk groups(P＜0.001),with 1-,3-,and 5-year AUC values of 0.610,0.668,and 0.665,respectively.The risk score was an independent prognostic factor(HR=11.261,95％CI:3.654-34.701,P＜0.001)and showed predictive efficacy in both early-stage(Ⅰ-Ⅱ,P=0.022)and advanced-stage(Ⅲ-Ⅳ,P=0.004)patients.GSEA revealed significant enrichment of Alzheimer's disease(P=0.003)and cell adhesion pathways(P=0.008)in high-risk groups.SNAI1 correlated positively with M1/M2 macrophage infiltration(r=0.45,P＜0.001),MDH1 associated with 12 immune cell types(|r|＞0.3,P＜0.05),and the risk score linked to CD8+T cells(r=0.38)and M2 macrophages(r=0.32)(both P＜0.001).Twenty-three immune checkpoints(e.g.,TNFRSF14,CD200R1)were differentially expressed between groups and survival-related(P＜0.05).High-risk patients exhibited elevated TIDE scores(P＜0.001),indicating enhanced immune suppression.Conclusion:This model provides a novel tool for LUSC prognosis assessment,but further clinical validation is required.
