Ginsenoside Rb1 Protects Oxidative Stress Damage and Apoptosis Induced by Palmitoic Acid in Human Umbilical Vein Endothelial Cells
10.13241/j.cnki.pmb.2025.17.004
- VernacularTitle:人参皂苷Rb1对PA诱导的HUVECs氧化应激损伤的保护作用机制
- Author:
Qing-li LI
1
;
Jun-qing GAO
1
;
Hong ZHANG
1
;
You-bin LIU
1
;
Zong-jun LIU
1
Author Information
1. 上海市普陀区中心医院心血管内科 上海 200062;上海中医药大学附属普陀医院心血管内科 上海 200062
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb1;
Palmitic acid;
Human umbilical vein endothelial cells;
Oxidative stress damage;
Mechanism of protective effect
- From:
Progress in Modern Biomedicine
2025;25(17):2749-2758
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To determine whether the Rb1 of ginsenoside has protective effects on PA induced oxidative stress in endothelial cells.Methods:Established a model of palmitic acid-induced oxidative stress injury in human umbilical vein endothelial cells(HUVECs).Using MTT assay,flow cytometry,fluorescent probe staining,and Western blot analysis to detect whether Rb1 of ginsenoside has effects on the cell viability,apoptosis rate,ROS and NO production,mitochondrial membrane potential,and the expression levels of related proteins.Results:MTT assay and flow cytometry revealed that ginsenoside Rb1 can reduce PA-induced apoptosis in HUVECs(P＜0.05).The mechanism may be related to the following two points:(1)reducing ROS production and increasing NO levels,thereby enhancing the antioxidant capacity of HUVECs;(2)regulating the expression of Bcl-2 family proteins,increasing the BCL-2/Bax ratio(P＜0.05),modulating mitochondrial membrane permeability,reducing cytochrome C release(P＜0.001),and decreasing Caspase protein activation(P＜0.01),thereby attenuating PA-induced apoptosis.Conclusion:After the stimulation with PA,ROS production in human umbilical vein endothelial cells increased while NO content and cell activity decreased,oxidative stress induced apoptosis in cells.By regulating the production of ROS and NOx stabilizing the mitochondrial transmembrane potential,reducing the leakage of cytochrome C,Ginsenoside Rb1 can reduce HUVECs apoptosis induced by PA.
