Short-term pancreatic cancer mouse model established by cancer cell inoculation and its in vivo imaging assessment
10.13267/j.cnki.syzlzz.2025.050
- VernacularTitle:小鼠癌细胞接种胰腺癌短期模型及其活体成像评估
- Author:
Yukun DU
1
;
Xiao CHEN
;
Xintong PAN
;
Ziqian LI
;
Tianqi WANG
;
Kaijun WANG
;
Yanan LI
Author Information
1. 西北妇女儿童医院药剂科,陕西 西安 710000
- Publication Type:Journal Article
- Keywords:
pancreatic cancer;
in vivo imaging technology;
normal immunized mice;
orthotopic vaccination model;
ectopic vaccination model
- From:
Practical Oncology Journal
2025;40(4):331-338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish orthotopic and ectopic pancreatic cancer models in C57BL/6N mice with normal immune function using in vivo imaging technology for visual characterization.Methods Orthotopic and ectopic pancreatic cancer models were established in Kunming mice by injecting a small volume of cell suspension containing firefly luciferase-expressing Panc02-luciferase pancreatic cancer cells into the head of the pancreas or the right axillary region.In vivo imaging technology was used to optimize the modeling method and timing in Kunming mice.Subsequently,the same method was applied to C57BL/6N mice using wild-type Panc02 pancreatic cancer cells to establish orthotopic and ectopic pancreatic cancer models with intact immune function.Key parameters,including body weight,inoculation positive rate,tumor growth time,tumor volume,and pathological characteristics across different organs,were compared be-tween the orthotopic and ectopic models in C57BL/6N mice to evaluate the applicability of these models.Results Both the small animal in vivo imaging experiments in Kunming mice and the tumor growth observation in C57BL/6N mice demonstrated that the construction periods for orthotopic and ectopic pancreatic cancer models were 20 days,with survival rates exceeding 90%.The inoculation positive rates in C57BL/6N mice were 92.3%for the orthotopic model and 78.6%for the ectopic model.On day 20 post-inoculation,the tumor volumes were(117.04±109.56)mm3 for the orthotopic model and(155.68±168.73)mm3 for the ectopic model,indicating high model success rates and consistent tumor growth.HE staining revealed pathological mitotic figures and poorly differentiated tumor tissues in both models of C57BL/6N mice,with no evidence of metastasis to other organs.Conclusions Orthotopic and ectopic pancreatic cancer models in immu-nocompetent mice were successfully developed in this study,mimicking early-stage pancreatic cancer characteristics.These models pro-vide a reliable platform for screening early diagnostic biomarkers and evaluating therapeutic interventions for pancreatic cancer.
