The effect of LCN2-mediated EGFR phosphorylation on inflammatory injury in human fallopian tube epi-thelial cells
10.3969/j.issn.1006-5725.2025.14.008
- VernacularTitle:LCN2介导EGFR磷酸化对人输卵管上皮细胞炎性损伤的影响
- Author:
Yaqiong XIA
1
;
Yue ZHU
;
Chengcheng JIANG
;
Hongping TAN
;
Yan ZHANG
;
Ling LIU
Author Information
1. 湖南中医药大学附属省直中医医院妇二科(湖南株洲 412000)
- Publication Type:Journal Article
- Keywords:
LCN2;
inflammatory damage of human fallopian tube epithelial cells;
EGFR;
EGFR phosphorylation
- From:
The Journal of Practical Medicine
2025;41(14):2174-2182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effect of lipocalin 2(LCN2)on epidermal growth factor receptor(EGFR)phosphorylation and its impact on inflammatory damage in human fallopian tube epithelial cells.Methods Human fallopian tube epithelial cells were isolated and a lipopolysaccharide(LPS)intervention was applied to establish an in vitro cell model.The cells were randomly assigned into the following groups:a blank control group(Control),a model group(Model),experimental groups(Model+si-LCN2 or Model+oe-LCN2),and negative control groups(Model+si-NC or Model+oe-NC).Changes in cell viability,apoptosis rates,inflam-matory levels,as well as the expression of EGFR mRNA,LCN2,EGFR,p-EGFR,and the ratio of p-EGFR/EGFR proteins were evaluated.Results Compared to the Model group,the Model+si-LCN2 group exhibited enhanced cell viability,a reduced apoptosis rate,and decreased expression of inflammatory factors(P＜0.05).Immunopre-cipitation analysis confirmed a direct interaction between LCN2 and EGFR.In comparison with the Model group,the Model+oe-LCN2 group demonstrated elevated levels of p-EGFR and the p-EGFR/EGFR ratio(P＜0.05),while no significant change was observed in total EGFR expression(P＞0.05).Conclusion Inhibition of LCN2-mediated EGFR phosphorylation enhances cell viability,reduces apoptosis,and mitigates inflammatory responses,thereby ameliorating LPS-induced inflammatory injury in human fallopian tube epithelial cells.
