Sequence determination and analysis of whole genome of enzootic nasal tumor virus of goats in Yunnan Province
10.16303/j.cnki.1005-4545.2025.08.07
- VernacularTitle:云南省山羊地方性鼻内肿瘤病毒全基因组序列的测定及分析
- Author:
Lichun XIE
1
;
Zixi LUO
1
;
Qian LI
1
;
Shulin MAI
1
;
Haiyan YAO
1
;
Xiao ZHANG
1
;
Shanshan QI
1
;
Guishu YANG
1
;
Gefen YIN
1
;
Yongneng LI
1
Author Information
1. 云南农业大学动物医学院,云南 昆明 650201
- Publication Type:Journal Article
- Keywords:
enzootic nasal tumor virus of goats;
whole genome;
sequence analysis;
evolutionary tree analysis
- From:
Chinese Journal of Veterinary Science
2025;45(8):1632-1641
- CountryChina
- Language:Chinese
-
Abstract:
To further investigate the molecular genetic characteristics of enzomatic nasal tumor vi-rus of goats(ENTV-2)in Yunnan Province,this study measured and analyzed the entire genome of ENTV-2 in Yunnan Province.The results showed that the complete genome sequence of the EN-TV-2 YN2023 strain(GenBank accession number:PP682590.1)was successfully obtained.The YN2023 strain has a total length of 7 307 bp and a typical structure of 5'-M5-gag-pro-pol-env-M3-3'.Whole genome sequence homology analysis showed that the nucleotide homology between YN2023 strain and 41 reference strains ranges from 85.3％to 95.5％.The whole genome evolution-ary tree indicates that the YN2023 strain is closely related to the prevalent strains in China,with certain genetic diversity and geographical clustering.The analysis of the gag gene evolutionary tree shows that the gag gene cluster of YN2023 strain is on a branch of the ENTV-2 gag gene,and YN2023 is clustered on the same small branch as enENTV-FJ1 and GDQY2017 strains,with the closest genetic relationship.The env gene evolutionary tree shows that YN2023 is on the same branch as GDQY2017,GDZJ2022,ENTV-2CHN1-6,ENTV-FJ1,and ENTV-FJ3,and is also on the same branch as GDQY2017,indicating a close genetic relationship.Recombination analysis showed that the YN2023 strain underwent a potential recombination event between breakpoint positions 6378-7478 bp,with the Chinese Chongqing strain enENTV-CQ1(OR669623.1)as the primary parent and the Chinese Sichuan strain BH(MT254062.1)as the secondary parent.This study enriches the genomic information of the ENTV-2 strain in Yunnan Province and provides data sup-port for the genetic variation of ENTV-2 in Yunnan Province.
