Structural and functional analysis of the NS2 protein of porcine hemagglutinating encephalomyelitis virus
10.16303/j.cnki.1005-4545.2025.09.04
- VernacularTitle:猪血凝性脑脊髓炎病毒NS2蛋白的结构与功能分析
- Author:
Ao ZHANG
1
;
Shaoqian MU
1
;
Yihan TIAN
1
;
Ruizhao QIU
1
;
Guoce FU
1
;
Junchao SHI
1
;
Feng GAO
1
;
Wen-qi HE
1
;
Deguang SONG
1
;
Zi LI
1
Author Information
1. 吉林大学动物医学学院,吉林长春 130062
- Publication Type:Journal Article
- Keywords:
PHEV;
NS2 protein;
bioinformatics analysis;
eukaryotic gene expression;
transcriptome sequencing;
structural function
- From:
Chinese Journal of Veterinary Science
2025;45(9):1843-1848,1887
- CountryChina
- Language:Chinese
-
Abstract:
Porcine hemagglutinating encephalomyelitis virus(PHEV)is one of the coronaviruses susceptible to swine populations.The non-structural protein 2(NS2)encoded by its genome is fre-quently deleted during the epidemic transmission of the virus,but its biological significance re-mains unclear.In order to explore the structure and function of the NS2 protein,this study utilized platforms such as ProtParam,TMHMM,NetPhos3.1,and ExPASy to analyze its physicochemical properties,spatial structure,genetic evolution,and post-translational modification characteristics.Meanwhile,the NS2 protein was expressed in eukaryotes and transcriptome sequencing was per-formed to clarify the biological processes it participates in.The results showed that the NS2 protein consists of 233 amino acids,with a molecular weight of 26.735 kDa,and a half-life of approximately 30 hours in mammals.It includes 13 phosphorylation sites,2 N-glycosylation sites,and 1 O-glyco-sylation site,with no signal peptide and strong hydrophilicity.The a-helix accounts for the highest proportion in NS2(43.78％),followed by random coils(36.05％).The homology of the NS2 pro-tein between the epidemic strains PHEV-CC14 and PHEV-JL/2008 in Northeast China is 99.57％.The NS2 protein is widely involved in the regulation of nerve-related functions,such as axon guid-ance and synaptic development.This study preliminarily clarified the biological function of the NS2 protein,providing a new perspective for understanding the pathogenic mechanism of PHEV.
