In vitro inhibitory and clinical application effect of Sophora flavescens,Philo-dendron extracts and copper sulfate on Trichomonas gallinae
10.16303/j.cnki.1005-4545.2025.09.13
- VernacularTitle:苦参、黄柏提取物和硫酸铜对鸽毛滴虫体外抑制作用与临床应用效果
- Author:
Yifei LONG
1
;
Liangming KUANG
1
;
Xingchen ZHAO
1
;
Ming GAO
1
;
Yihong SUN
1
;
Zifan WANG
1
;
Shuo ZHOU
1
;
Wei WANG
1
Author Information
1. 仲恺农业工程学院,广东 广州 510225
- Publication Type:Journal Article
- Keywords:
sophora flavescens;
philodendron;
copper sulfate;
trichomonas gallinaes;
metronidazole
- From:
Chinese Journal of Veterinary Science
2025;45(9):1918-1926
- CountryChina
- Language:Chinese
-
Abstract:
Aimed to find a safe and effective drug to replace nitroimidazole drugs in aquaculture pro-duction for the prevention and treatment of Trichomoniasis in pigeons,which can improve the eco-nomic benefits of meat pigeon breeding and ensure food safety.Firstly,Trichomonas was isolated and cultured from the crop of diseased pigeons and identified.After stable passage,a quantitative method for in vitro detection of Trichomonas was established by combining an automated cell counter and quantitative real-time PCR technology.To prepared the drug,powders of Sophora fla-vescent and Philodendron were made into herbal water extracts(SFPA)and mixed with copper sulfate(CS)solution.Then added the drug to the culture medium of Trichomonas to determine the effective concentration of it.A total of 135 pairs each of Silver King and Mimas breeding pigeons in the same laying period were selected and randomly divided into three groups,with 6 replicates in each group and 15 pairs of breeding pigeons in each replicate.Four days before brooding,the three groups were fed with 200 mL of pure water,0.5 g/L metronidazole(MDZ)solution,and a mixed solution of 30 g/L SFPA and 0.5 g/L CS,respectively.The feeding experiment lasted for 26 d.Results showed that the mixed solution of SFPA and CS had a significant killing effect on Trichomonas in vitro(P＜0.05).Feeding the drug to breeding pigeons significantly reduced the in-fection rate of breeding pigeons by Trichomonas(P＜0.05).The drug had no significant effect on the serum biochemical indexes,antioxidant properties,immunoglobulin levels of breeding pigeons,the average cage weight,immune organ indexes,meat quality and slaughter performance of squabs(P＞0.05).The results suggested that adding SFPA and CS to pigeons can effectively prevent and treat Trichomoniasis and improve production performance.It can replace nitroimidazole drugs without affecting the immune level of breeding pigeons and the weight,immune level,slaughter performance and meat quality of squabs,thereby reduce drug residues in poultry products and en-hance the food safety.
