Preparation of the neutralizing monoclonal antibody against foot-and-mouth dis-ease virus serotype O and identification of antigenic epitope
10.16303/j.cnki.1005-4545.2025.09.05
- VernacularTitle:口蹄疫病毒O型中和单克隆抗体的制备及其抗原表位鉴定
- Author:
Yue SUN
1
;
Junjun SHAO
;
Shandian GAO
;
Guangqing ZHOU
;
Huichen GUO
;
Huiyun CHANG
;
Yong ZHANG
;
Xingxu ZHAO
;
Wei LIU
Author Information
1. 甘肃农业大学动物医学院,甘肃兰州 730070;甘肃省动物生殖生理与繁殖调控重点实验室,甘肃兰州 730070
- Publication Type:Journal Article
- Keywords:
foot-and-mouth disease virus;
serotype;
VP1;
neutralizing antibody;
epitope identification
- From:
Chinese Journal of Veterinary Science
2025;45(9):1849-1856
- CountryChina
- Language:Chinese
-
Abstract:
Although no cross protection was observed between different serotypes of foot-and-mouth disease virus(FMDV),there were cross-reactivity between different serotypes of antibodies produced after vaccination,the aim of this paper was to prepare the neutralizing monoclonal anti-body against Foot-and-mouth disease virus(FMDV)serotype O,and to develop the method to dis-tinguish antibody against FMDV serotype O and A based on mAb.The inactivated FMDV serotype O was used as antigen in mAb production,a series of GST fusion overlapping peptides and trun-cated peptides expressed in Escherichia coli were used to identify antigenic epitope recognized by monoclonal antibodies.In order to verify feasibility of the screened monoclonal antibodies in diag-nosis,20 positive serum of FMDV serotype O and A,20 negative serum with known background were detected by blocking ELISA.Results were as follows:five monoclonal antibodies were suc-cessfully screened.The five monoclonal antibodies showed good reactivity with FMDV serotype O,but did not react with FMDV serotype A by Western blot and IFA,these mAbs showed neutrali-zing ability to FMDV/O/MY98/GZBY/2013 by VNT.The same epitope was identified by five monoclonal antibodies,the minimum epitope was145 RGDLQVLA152,Arg145 and Gln149 were key a-mino acids of the epitope.Sequence alignment analysis revealed that the identified epitopes were conserved among most of O type FMDV strains,but Gln149 was mutated among all A,Asia 1 and SAT1-3 type FMDV strains.The mAb-8C5D3 distinguished between antibody of FMDV serotype O and FMDV serotype A by blocking ELISA.The results provided materials for development of O type FMDV antibody detection kit and evaluation of vaccine immune effect.
