Characterization of Yersinia enterocolitis in patients with diarrhea in a district of Beijing
10.3969/j.issn.1002-2694.2025.00.107
- VernacularTitle:北京市某区腹泻患者感染小肠结肠炎耶尔森菌病原特征分析
- Author:
Yu-wei LIU
1
;
Hai-rui WANG
;
Yan-chun ZHANG
;
Shou-fei LI
;
Luo-tong WANG
;
Miao WANG
;
Ai-xia YAN
;
Ying LI
;
Mao-jun ZHANG
Author Information
1. 北京市顺义区疾病预防控制中心,北京 101300;北京市顺义区疾病预防控制中心微生物感染性疾病检测工作站,北京 101300
- Publication Type:Journal Article
- Keywords:
Yersinia enterocolitica;
diarrhea;
drug resistance;
whole genome sequencing
- From:
Chinese Journal of Zoonoses
2025;41(6):609-616
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at providing basic data for the control and prevention of Yersinia enterocolitica(Ye)infections.Ye isolates from stool samples collected from patients with diarrhea in a Beijing district between January 2019 and June 2024 were studied.Basic patient information and stool samples were collected,and quantitative polymerase chain reaction(qPCR)was applied to enriched cultures.Further analyses included virulence gene detection,whole-genome sequencing,and drug resistance detection.The detection rate of Ye was 0.76%(11/1 439),according to culture methods,thus yielding 12 Ye strains from distinct patients:11 isolated during the study period and 1 from 2017.The 12 Ye positive patients were 6-41 years of age,and their clinical presentations predominantly featured watery stools(66.67%,8/12)and loose stools(33.33%,4/12).The frequencies of nausea,vomiting,and fever were 41.67%(5/12),41.67%(5/12),and 8.33%(1/12),respectively.The drug resistance rates of Ye to TET,AMP,and NAL were 50.00%(6/12),33.33%(4/12),and 25.00%(3/12),respectively.One Ye strain exhibited multidrug resistance to ETP,MEM,TET,CIP,NAL,and AMP.According to qPCR detection of five common virulence genes,two Ye strains were identified as ystA+/ystB-type(ystA+/ystB-/ail+/yadA+/virF+),whereas ten strains were identified as ystA-/ystB+type(ystA-/ystB+/ail-/yadA-/virF-).VFDB database analysis based on genome sequences indicated that 12 Ye strains carried an average of 11 key virulence genes associated with adhesion,invasion,protease activity,and flagellar movement,and predicted 106 virulence genes and 12 virulence gene profiles.Only the two ystA+/ystB-Ye strains contained elements related to the TTSS and ABC transporter function.Detection of ystA-/ystB+Ye in stool isolation and culture of diarrhea cases might potentially have been missed in some cases,thus highlighting the importance of fluorescence PCR screening of fecal growth solutions to enhance isolation efficiency.Moreover,our findings revealed the genetic diversity of Ye isolated from diarrhea cases,thereby indicating the presence of multiple types of virulence genes within this pathogen.
