Construction and identification of a sizeable naive human Fab phage display antibody library
10.3760/cma.j.cn112338-20240801-00468
- VernacularTitle:大容量天然人源Fab噬菌体展示抗体库的构建及鉴定
- Author:
Yakun ZHAO
1
;
Xiaoyue WEI
;
Fanliang MENG
;
Wentao LIU
;
Jiaming FAN
;
Lijin LONG
;
Wanting WANG
;
Jianling CHEN
;
Jianzhong ZHANG
;
Lihua HE
;
Liyong LIU
;
Rui ZHAO
;
Di SUN
;
Xuezhen YUAN
;
Xiaomei YAN
Author Information
1. 中国医科大学公共卫生学院卫生统计学教研室，沈阳 110122
- Publication Type:Journal Article
- Keywords:
Phage display antibody library;
Antigen-binding fragment;
Naive antibody library
- From:
Chinese Journal of Epidemiology
2025;46(2):288-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a sizeable naive human Fab phage display antibody library to screen high-affinity specific antibodies in vitro. Methods:Total RNA was extracted from peripheral blood mononuclear cells (PBMCs) of 126 healthy individuals, subsequently reverse-transcribed into cDNA, and used as a template. PCR amplification was performed to obtain the V H from IgG, IgM and light chain κ, λ, separately, with the initial PCR products serving as templates for a second round of PCR. Overlap extension PCR was employed to generate fragments of the κ and λ light chains. These fragments were ligated with the phage vector pNC3, which harbors the variable region 1 of the heavy chain, to construct a recombinant phage plasmid. This plasmid was then electroporated into competent Escherichia Coli TG1 cells to establish a naive human Fab phage display antibody library. One hundred clones were randomly selected for identification and sequencing, and antibody gene polymorphisms were analyzed using the IMGT database and MAFFT software. Recombinant α-hemolysin from Staphylococcus aureus was utilized to screen Fab antibody fragments through biopanning of the antibody library, followed by random selection of phage ELISA-identified clones. The positive clones (antigen A450∶blank control A450≥2.1) were sequenced. Results:Two large naive Fab phage display antibody libraries were successfully constructed, in which the capacity of κ and λ chain antibody libraries were 1.25×10 11 and 1.54×10 11, respectively. The titers for two antibody libraries were 6.04×10 13 CFU/ml and 3.50×10 13 CFU/ml. The positive transformation insertion rates for κ and λ chain antibody libraries were 96% (96/100) and 100% (100/100), respectively. Sequence analysis revealed that all antibody sequences were unique. The amino acid sequences in the skeletal region were relatively conserved. In contrast, significant variations in the length of the complementarity determining region (CDR) were found, and the diversity of amino acid sequence of the complementary determining region was high, especially the CDR3. Analysis using the IMGT database indicated that the sequences exhibited a broad distribution across variable-diversity-joining gene families. After six rounds of panning, specific phage antibodies enrichment targeting α-hemolysin were achieved. A total of 142 monoclonal antibodies were sequenced, yielding 8 distinct Fab antibody sequences. Conclusion:This study successfully constructed two naive human Fab phage display antibody libraries with large capacity and good diversity, which can be used for screening human antibodies for serum epidemiology.
