Antiviral effect of Lopinavir against porcine hemagglutinated encephalomyelitis virus
10.16303/j.cnki.1005-4545.2025.08.18
- VernacularTitle:洛匹那韦抗猪血凝性脑脊髓炎病毒感染的效果评价
- Author:
Yanfang YU
1
;
Yihan TIAN
1
;
Jianwei WEI
1
;
Zi LI
1
;
Junchao SHI
1
;
Feng GAO
1
;
Wenqi HE
1
Author Information
1. 吉林大学 动物医学学院,吉林 长春 130062
- Publication Type:Journal Article
- Keywords:
PHEV;
Lopinavir;
antiviral drug;
coronavirus infection
- From:
Chinese Journal of Veterinary Science
2025;45(8):1720-1726
- CountryChina
- Language:Chinese
-
Abstract:
This study explores the antiviral effects of Lopinavir on porcine hemagglutinating en-cephalomyelitis virus(PHEV)in vitro and in vivo.Using PHEV-infected N2a cells as an in vitro experimental model,the impact of varying concentrations of Lopinavir on PHEV replication was analyzed through Western blot and qRT-PCR techniques.The results demonstrated that Lopinavir was beneficial to PHEV replication at low-concentration,but as the concentration increased,Lopi-navir began to exert an inhibitory effect,with the most pronounced effect observed at a concentra-tion of 8 μmol/L.PHEV-infected 3-week-old male BALB/c mice were utilized in vivo experi-ments,with Lopinavir(10 mg/kg)administered intragastrically three days post-infection.Follow-ing the onset of illness in the control group,all mice were euthanized,and brain tissues were col-lected for histopathological examination.The findings indicated that Lopinavir significantly reduced the distribution of PHEV and ameliorated the pathological damage in brain tissue,and prolonged the survival time of the mice.In conclusion,Lopinavir exhibits an antiviral effect against PHEV both in vitro and in vivo,offering a theoretical basis for the prevention and treatment of PHEV in-fections in clinical practice.
