Improvement of immune function and intestinal flora in immunosuppressed mice by Dilong fermentation
10.16303/j.cnki.1005-4545.2025.08.17
- VernacularTitle:地龙发酵物对免疫抑制小鼠免疫功能和肠道菌群的改善作用
- Author:
Yuqing CUI
1
;
Yishan LIU
;
Xingliang PAN
;
Yongzhan BAO
;
Wanyu SHI
;
Liting CAO
Author Information
1. 西南大学动物医学院,重庆荣昌 402460;西南大学中兽医研究所,重庆荣昌 402460
- Publication Type:Journal Article
- Keywords:
Dilong fermentation;
immunity;
gut microbiota;
Lactobacillus
- From:
Chinese Journal of Veterinary Science
2025;45(8):1713-1719
- CountryChina
- Language:Chinese
-
Abstract:
Thirty SPF Kunming male mice aged 6-8 weeks were randomly divided into five groups:the control group,the model group,and the low,medium,and high dose groups of Dilong.Except for the control group,the other mice were continuously injected intraperitoneally with 80 mg/kg cyclophosphamide for 3 days to prepare an immunosuppressive model.After the model-ing was completed,the control group and the model group were given an equal amount of physio-logical saline by gavage,while the low,medium,and high dose Dilong groups were given 100,200,and 400 mg/kg of Dilong fermented material by gavage,respectively.The treatment lasted for 14 days,and thymus,spleen,blood,and intestinal contents were collected 2 hours after the last admin-istration.The results showed that cyclophosphamide caused a weight loss in mice during the exper-iment,reduced thymus index,white blood cells,lymphocytes,granulocytes,immunoglobulin levels in the blood,and disrupted gut microbiota.Dilong fermented material can effectively improve the decrease in body weight and thymus index of mice,increase the levels of immune cells and immu-noglobulin in the blood,improve the diversity and richness of intestinal microbiota,and increase the abundance of beneficial bacteria.Dilong fermented can effectively alleviate the immune suppres-sion and intestinal microbiota disorder caused by cyclophosphamide in mice.Considering all fac-tors,the optimal dosage is 200 mg/kg.
