Genetic diversity,virulence factors,and antibiotic resistance of Listeria monocytogenes from livestock and poultry meat and clinical samples in Weifang during 2020-2023
10.3969/j.issn.1002-2694.2025.00.105
- VernacularTitle:2020-2023年潍坊市畜禽肉和临床样本中单增李斯特菌的遗传多样性、毒力因子和抗生素耐药性研究
- Author:
Yi-meng WANG
1
;
Ming-ming YU
;
De-hui LIU
;
Xiao-yan HAO
;
Li-dan JIANG
;
Zhi-peng REN
Author Information
1. 潍坊市疾病预防控制中心,潍坊 261061;山东省医药卫生重点学科,潍坊 261061
- Publication Type:Journal Article
- Keywords:
Listeria monocytogenes;
antimicrobial susceptibility testing;
whole-genome sequencing;
resistance genes;
virulence genes
- From:
Chinese Journal of Zoonoses
2025;41(6):629-635
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at understanding the antimicrobial resistance patterns,virulence characteristics,and phyloge-netic relationships of foodborne Listeria monocytogenes in Weifang.A total of 67 strains of Listeria monocytogenes were isolated from livestock,poultry meat,and clinical samples in Weifang between 2020 and 2023.The susceptibility of these isolates was determined through broth microdilution.Whole-genome sequencing and genetic characterization of these isolates were conducted.The 67 strains were divided into 12 STs,among which ST121,ST8,ST9,and ST87 predominated(76.12%).Eight groups of closely related strains were identified through cgMLST typing.Three Listeria pathogenicity islands and two genomic islands were identified in all strains:100%of the strains carried LIPI-1,5.97%carried LIPI-3,14.93%carried LIPI-4,2.99%carried LGI-2,and 4.48%of the strains carried LGI-3.No antibiotic resistance genes were found in any strains.All isolates were susceptible to ampicillin,penicillin,merope-nem,trimethoprim-sulfamethoxazole,and vancomycin.Five isolates were resistant to tetracycline,and three strains of ST87,one strain of ST8,one strain of ST224,and two strains of ST87 were simultaneously resistant to erythromycin.The tet(M)tetracycline re-sistance genes and msr(D)and mef(A)erythromycin resistance genes from three strains of ST87 and one strain of ST8 were carried by a phage similar to phi1605 in Erysipelothrix,with>95%identity.The tet(M)gene from the ST224 isolates was carried by a transposon similar to Tn5801_B15 in Enterococcus faecalis,with>95%identity.Drug-resistant strains of Listeria monocytogenes were found in livestock and poultry meat on sale in Weifang,particularly strains of type ST87 and ST224 simultaneously carrying highly pathogenic virulence islands,thus posing a threat to food safety and public health.These findings therefore warrant attention from relevant depart-ments and strengthened monitoring efforts.
